Bailey M J, Cockayne A, Penn C W
Department of Microbiology, University of Birmingham, UK.
J Gen Microbiol. 1987 Jul;133(7):1805-13. doi: 10.1099/00221287-133-7-1805.
A suitable immunization protocol for the stimulation of a murine antibody response to the axial filament polypeptides of Treponema pallidum was established. A range of monoclonal antibodies (Mabs) specific for different epitopes of the major axial filament polypeptide (37 kDa) was generated which demonstrated diversity in their ability to react with other treponemal species. Immunogold electron microscopy located the 37 kDa antigen on the surface of the axial filament structure. The early appearance of specific antibody to this polypeptide in infected man and rabbit indicates that such Mabs are potentially useful for the diagnosis of early syphilis.
已建立了一种合适的免疫方案，用于刺激小鼠对梅毒螺旋体轴丝多肽产生抗体反应。制备了一系列针对主要轴丝多肽（37 kDa）不同表位的单克隆抗体（Mabs），这些抗体在与其他密螺旋体物种反应的能力上表现出多样性。免疫金电子显微镜将37 kDa抗原定位在轴丝结构的表面。在感染的人和兔子中这种多肽特异性抗体的早期出现表明，此类单克隆抗体可能对早期梅毒的诊断有用。
