Engelkens H J, Kant M, Onvlee P C, Stolz E, van der Sluis J J
Department of Dermatology and Venereology, Erasmus University Rotterdam, The Netherlands.
Genitourin Med. 1990 Oct;66(5):367-73. doi: 10.1136/sti.66.5.367.
The use of Percoll-purified treponemes in an assay similar to the Treponema pallidum Immobilisation test demonstrated that immobilisation of purified treponemes by seronegative normal human serum proceeded at a much higher rate than that of unpurified treponemes. This suggests that the removal of the testicular extract makes the treponemes more vulnerable to this action. A preincubation of the purified treponemes with the testicular extract from infected or uninfected testes delayed their rate of immobilisation to that demonstrated by the unpurified treponemes. This showed that substances produced during the infection are probably not responsible for the delay in immobilisation. Discrimination between the classical and the alternative pathway of complement activation, studied by the ethylene glycol-bis (beta-aminoethyl ether) N,N,N',N'-tetraacetic acid (EGTA) method, showed that the classical pathway was responsible for the rapid immobilisation of the purified treponemes. However, the slow immobilisation in the EGTA-serum samples suggested a minor role of the alternative pathway in the immobilisation of the purified treponemes. Since the testicular extracts exerted an anti-complement activity, it needs to be investigated whether the protection offered to the purified treponemes by the testicular extracts is based on their deteriorating effect on the classical complement pathway or is due to a re-establishment of the protective cover around the treponemes.
在一项类似于梅毒螺旋体固定试验的检测中使用Percoll纯化的密螺旋体，结果表明，血清阴性的正常人血清对纯化密螺旋体的固定速度比未纯化的密螺旋体快得多。这表明去除睾丸提取物会使密螺旋体更容易受到这种作用的影响。将纯化的密螺旋体与感染或未感染睾丸的睾丸提取物预孵育，会使其固定速度延迟至未纯化密螺旋体所显示的速度。这表明感染期间产生的物质可能与固定延迟无关。通过乙二醇双（β-氨基乙基醚）N,N,N',N'-四乙酸（EGTA）方法研究补体激活的经典途径和替代途径之间的差异，结果表明经典途径是纯化密螺旋体快速固定的原因。然而，EGTA-血清样本中的缓慢固定表明替代途径在纯化密螺旋体的固定中起次要作用。由于睾丸提取物具有抗补体活性，因此需要研究睾丸提取物对纯化密螺旋体的保护作用是基于其对经典补体途径的破坏作用，还是由于密螺旋体周围保护覆盖层的重新建立。
