Nakthong Prangthip, Kondo Takeshi, Chailapakul Orawon, Siangproh Weena
Electroanalytical and Imaging Sensor Research Group (EISRG), Department of Chemistry, Faculty of Science, Srinakharinwirot University, Sukhumvit 23, Wattana, Bangkok 10110, Thailand.
Anal Methods. 2020 Nov 28;12(44):5407-5414. doi: 10.1039/d0ay01443e. Epub 2020 Oct 30.
The high requirement for food quality control has inspired the creation of high-performance sensing, cost-effectiveness, and ease to use. Therefore, the aim of this work is to develop nonenzymatic electrochemical platforms for direct detection of histamine using unmodified screen-printed graphene electrodes (SPGEs) for their applications such as evaluation of fish freshness. In alkaline media (0.2 M NaOH), unmodified SPGEs showed a very low oxidation potential of histamine at +0.58 V (vs. Ag/AgCl) avoiding perturbations from other biogenic amines. The developed method offers an excellent selectivity, sensitivity (a limit of detection (at 3SD/slope) of 0.62 mg L) and wide working linear range (5-100 mg L) for histamine detection. In addition, the proposed method was successfully applied to detect histamine in canned fish samples with recovery values ranging from 90.72% to 101.21%. Therefore, this newly proposed method is promising as an alternative choice for the determination of histamine in fish samples and related food products.
对食品质量控制的高要求激发了高性能传感、成本效益和易用性的创新。因此，这项工作的目的是开发用于直接检测组胺的非酶电化学平台，使用未修饰的丝网印刷石墨烯电极（SPGE），用于评估鱼类新鲜度等应用。在碱性介质（0.2 M NaOH）中，未修饰的SPGE对组胺显示出非常低的氧化电位，为+0.58 V（相对于Ag/AgCl），避免了其他生物胺的干扰。所开发的方法对组胺检测具有出色的选择性、灵敏度（检测限（3SD/斜率）为0.62 mg/L）和宽工作线性范围（5-100 mg/L）。此外，该方法成功应用于检测罐装鱼样品中的组胺，回收率在90.72%至101.21%之间。因此，这种新提出的方法有望成为测定鱼样品和相关食品中组胺的替代选择。
