Munir Muhammad Abdurrahman, Badri Khairiah Haji, Heng Lee Yook, Inayatullah Ahlam, Nurinda Eva, Estiningsih Daru, Fatmawati Annisa, Aprilia Veriani, Syafitri Nur
Department of Pharmacy, Faculty of Health Science, Alma Ata University, Daerah Istimewa Yogyakarta, Bantul 55183, Indonesia.
Department of Chemical Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Malaysia.
ACS Omega. 2022 Feb 10;7(7):5982-5991. doi: 10.1021/acsomega.1c06295. eCollection 2022 Feb 22.
Histamine is an important substance that can be applied as a parameter for allergic reactions and food freshness. This study develops a method to produce a histamine sensor based on electrodes modified using polyurethane-LiClO. A sensor method was developed where this sensor was produced from polyurethane. The application of 4,4'-diphenylmethane diisocyanate (hard compound) and palm kernel oil-based monoester polyol (soft compound) to produce polyurethane (PU) based on bio-polyol. The addition of lithium perchlorate (LiClO) was done in order to increase the conductivity of PU. The oxidation process was detected using cyclic voltammetry, whereas the electrochemical impedance spectroscopy was used to analyze the conductivity of the polymer. The polyurethane-LiClO was attached on a screen-printed electrode (SPE) within 45 min. Moreover, the 1% LiClO-PU-SPE presented satisfactory selectivity for the detection of histamine in the pH 7.5 solution. The LiClO-PU-SPE presented a good correlation coefficient ( = 0.9991) in the range 0.015-1 mmol·L. The detection limit was 0.17 mmol·L. Moreover, the histamine concentration of mackerel samples was detected by the PU-SEP-LiClO. Several amine compounds were chosen to study the selectivity of histamine detection using SPE-PU-LiClO. The interference was from several major interfering compounds such as aniline, cadaverine, hexamine, putrescine, and xanthine. The technique showed a satisfactory selective analysis compared to the other amines. A satisfactory recovery performance toward varying concentrations of histamine was obtained at 94 and 103% for histamine at 0.01 and 0.1 mmol·L, respectively. The application of PU-SEP-LiClO as an electrochemical sensor has a great prospect to analyze histamine content in fish mackerel as a consequence of PU-SEP-LiClO having good selectivity and simplicity.
组胺是一种重要物质，可作为过敏反应和食物新鲜度的一个参数。本研究开发了一种基于用聚氨酯 - 高氯酸锂改性的电极来制备组胺传感器的方法。开发了一种传感器方法，其中该传感器由聚氨酯制成。应用4,4'-二苯基甲烷二异氰酸酯（硬化合物）和基于棕榈仁油的单酯多元醇（软化合物）来制备基于生物多元醇的聚氨酯（PU）。添加高氯酸锂（LiClO）是为了提高PU的导电性。使用循环伏安法检测氧化过程，而用电化学阻抗谱分析聚合物的导电性。聚氨酯 - 高氯酸锂在45分钟内附着在丝网印刷电极（SPE）上。此外，1% LiClO - PU - SPE在pH 7.5溶液中对组胺的检测具有令人满意的选择性。LiClO - PU - SPE在0.015 - 1 mmol·L范围内呈现出良好的相关系数（= 0.9991）。检测限为0.17 mmol·L。此外，用PU - SEP - LiClO检测了鲭鱼样品中的组胺浓度。选择了几种胺类化合物来研究使用SPE - PU - LiClO检测组胺的选择性。干扰来自几种主要的干扰化合物，如苯胺、尸胺、六亚甲基四胺、腐胺和黄嘌呤。与其他胺类相比，该技术显示出令人满意的选择性分析。对于0.01和0.1 mmol·L的组胺，分别获得了94%和103%的对不同浓度组胺的令人满意的回收率。由于PU - SEP - LiClO具有良好的选择性和简便性，将其作为电化学传感器应用于分析鲭鱼中的组胺含量具有很大的前景。
