Federal State Institution Federal Research Centre Fundamentals of Biotechnology of the Russian Academy of Sciences, Prospect 60-letiya Oktyabrya, 7/2, 117312 Moscow, Russia.
Department of Biochemistry and Cell Physiology, Voronezh State University, Universitetskaya pl., 1, Voronezh 394018, Russia.
Int J Syst Evol Microbiol. 2020 Dec;70(12):6373-6380. doi: 10.1099/ijsem.0.004544.
A novel obligately anaerobic spirochete strain K2 was isolated from bottom marine sediments at Crater Bay of Yankicha Island (Kuril Islands, Russia). Strain K2 had helical shape and Gram-negatively stained. The optimal growth conditions were as follows: the optimum temperature was 28-30 °C with range 5-34 °C; optimal pH at 7.0-7.5 with range of 6.8-8.5; NaCl optimum at 3-3.5 % (w/v) and range of 1-7 % (w/v). Strain K2 was catalase- and oxidase-negative. Glucose fermentation products were acetate, lactate, ethanol, CO, H. The major fatty acids were C, -C, -C C DMA, -C DMA. The G+C content of genomic DNA was 43.2 mol%. Phylogenetic analyses of 16S rRNA genes showed that strain K2 belonged to the genus of the family . The 16S rRNA gene sequence similarity of strain K2 and DSM 2029 and DSM 104770 was 96 and 94 %, respectively. Based on the results of our study, we propose the name sp. nov.; type strain K2 (=DSM 16308=VKM B-3266). Also, the taxonomic status of was revised: 16S rRNA genes sequence showed less than 89 % similarity to nearest phylogenetic neighbours. Therefore, we proposed to separate this species into a novel genus gen. nov., comb. nov.
从俄罗斯千岛群岛扬奇卡岛克拉特湾的海底沉积物中分离到一株新型专性厌氧螺旋体菌株 K2。菌株 K2 呈螺旋形，革兰氏阴性染色。最佳生长条件如下：最佳温度为 28-30°C，范围为 5-34°C；最佳 pH 值为 7.0-7.5，范围为 6.8-8.5；NaCl 最佳浓度为 3-3.5%（w/v），范围为 1-7%（w/v）。菌株 K2 过氧化氢酶和氧化酶均为阴性。葡萄糖发酵产物为乙酸盐、乳酸盐、乙醇、CO、H。主要脂肪酸为 C、-C、-C C DMA、-C DMA。基因组 DNA 的 G+C 含量为 43.2mol%。16S rRNA 基因的系统发育分析表明，菌株 K2 属于 属的一个种。菌株 K2 与 DSM 2029 和 DSM 104770 的 16S rRNA 基因序列相似性分别为 96%和 94%。基于我们的研究结果，我们提出了一个新种的名称 sp. nov.；模式菌株 K2（=DSM 16308=VKM B-3266）。此外，还修订了 的分类地位：16S rRNA 基因序列与最近的系统发育近邻的相似性小于 89%。因此，我们建议将该物种分离到一个新的属 属，新组合。
