Robberson D L, Wilkins C E, Clayton D A, Doda J N
Nucleic Acids Res. 1977;4(5):1315-38. doi: 10.1093/nar/4.5.1315.
Exhaustive EcoRI digests of circular dimer mitochondrial DNA (mtDNA) from mouse cell lines LD and LDTK- yield two major fragments whose average lengths are slightly smaller than the corresponding fragments of circular monomer mtDNA from mouse LA9 and LMTK- cells. A third fragment approximately 400 nucleotide pairs in length is frequently produced in less than molar yield. Exhaustive EcoRI digests of circular dimer mtDNA from human acute myelogenous leukemic leucocytes yield three major fragments. The presence of mtDNA resistant to cleavage as well as fragments of intermediate sizes indicatesmicroheterogeneity in the genomic positions of EcoRI recognition sequences in both mouse and human circular dimer mtDNA. Analysis of the distribution averages of circular contour lengths indicates microheterogeneity in the sizes of mouse LD and human mtDNAs. The denatured-renatured EcoRI fragments frequently contain a small loop(s) of single-strand DNA as would occur for deletion(s) or addition(s) of single-strand DNA as would occur for deletion(s) or addition(s) of nucleotide sequences in some of the circular dimer molecules.
对小鼠细胞系LD和LDTK - 的环状二聚体线粒体DNA（mtDNA）进行彻底的EcoRI酶切，产生两个主要片段，其平均长度略小于来自小鼠LA9和LMTK - 细胞的环状单体mtDNA的相应片段。还经常产生一个长度约为400个核苷酸对的第三个片段，其产量低于摩尔产量。对来自人类急性髓性白血病白细胞的环状二聚体mtDNA进行彻底的EcoRI酶切，产生三个主要片段。存在抗切割的mtDNA以及中等大小的片段，表明小鼠和人类环状二聚体mtDNA中EcoRI识别序列的基因组位置存在微异质性。对环状轮廓长度分布平均值的分析表明小鼠LD和人类mtDNA的大小存在微异质性。变性 - 复性的EcoRI片段经常包含一个小的单链DNA环，这在某些环状二聚体分子中核苷酸序列缺失或添加时会出现单链DNA的缺失或添加情况。
