Key Laboratory of Adaptation and Evolution of Plateau Biota, Qinghai Provincial Key Laboratory of Animal Ecological Genomics, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, Qinghai, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Key Laboratory of Adaptation and Evolution of Plateau Biota, Qinghai Provincial Key Laboratory of Animal Ecological Genomics, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, Qinghai, China.
Anim Reprod Sci. 2020 Dec;223:106624. doi: 10.1016/j.anireprosci.2020.106624. Epub 2020 Oct 13.
In the present study, there were 128, 263, and 139 differentially expressed genes (DEGs) from the ovary, pituitary, and hypothalamus, respectively, with the FecB B+/FecB ++ genotype. Results indicated that there was a synchronized, and differential increase in mRNA transcript abundances for genes encoding for ovarian steroidogenesis-signaling proteins in the ovary, TGF-β-signaling in hypothalamus, cAMP-signaling-transduction, and dopaminergic-synapse pathways in the pituitary. The values for these variables were associated with mean prolificacy in indigenous sheep and results provided indications for candidate regulator proteins that coordinate processes related to prolificacy in ewes. Furthermore, the results when there was evaluation of the FecB-genotype-specific co-expression modules implied that CYP17 was the hub gene connecting the pathways and for modulation of folliculogenesis and ovulation in the ewe with the FecB B+ genotype.
在本研究中，FecB B+/FecB ++ 基因型的卵巢、垂体和下丘脑分别有 128、263 和 139 个差异表达基因（DEGs）。结果表明，卵巢中编码卵巢甾体生成信号蛋白、下丘脑 TGF-β 信号、垂体 cAMP 信号转导和多巴胺能突触途径的基因的 mRNA 转录丰度呈同步且差异增加。这些变量的值与本地绵羊的平均繁殖力相关，结果为协调母羊繁殖力相关过程的候选调节蛋白提供了指示。此外，当评估 FecB 基因型特异性共表达模块时，结果表明 CYP17 是连接途径的枢纽基因，用于调节具有 FecB B+ 基因型的母羊的卵泡发生和排卵。
