Kumar S, Mishra A K, Kolte A P, Arora A L, Singh D, Singh V K
Animal Biotechnology Section, Central Sheep and Wool Research Institute, Avikanagar, Rajasthan, India.
Anim Reprod Sci. 2008 May;105(3-4):319-31. doi: 10.1016/j.anireprosci.2007.03.012. Epub 2007 Mar 27.
The present study was conducted to evaluate the effects of FecB genotypes on body weight, average daily gain (ADG), ewe's productivity efficiency (EPE) and litter size in FecB introgressed GarolexMalpura (GM) crossbred sheep. A total of 235 GM lambs were selected randomly and screened for FecB mutation using forced RFLP-PCR. The majority (69.8%) of GM individuals were carriers (BB and B+) for the FecB mutation and frequency of the FecB allele was about 0.40. The FecB genotypes were significantly (P<0.01) associated with the lamb's body weights from birth to 12 months of age. The generation wise (F(1), F(2) and F(3)), lamb's body weight did not differ significantly at birth, 6 and 12 months of the age, while it differed significantly (P<0.05) at 3 and 9 months of age. The ADG1 (0-3 months) was significantly associated (P<0.05), but not the ADG2 (3-6 months) and ADG3 (6-12 months) between genotypes. Type of birth and sex significantly (P<0.01) affected the body weight from birth to 12 months of age; and body weight of single born lambs was significantly higher (P<0.01) than that of twins and triplets from birth to 12 months of age. Type of birth significantly (P<0.01) affected the ADG1, but had no significant effect on ADG2 and ADG3. Year of birth did not affect the birth and weaning weights, but it significantly affected (P<0.01) the body weight and ADG's after weaning ages. The EPE was affected significantly (P<0.01) by the FecB genotypes at birth, 3 and 12 months of age. The EPE of B+ and BB ewes were 7.86 kg (36.9%) and 2.32 kg (10.9%) higher as compared to ++ ewes at 12 months of age, respectively. The mean litter size of BB ewes (2.17+/-0.24) was significantly higher (P<0.01) than that of B+ ewes (1.73+/-0.04) and ++ ewes (1.03+/-0.23). The present study indicated that the body weight and ADG of carrier lambs (BB and B+) was comparatively lower than that of non-carriers (++), while EPE of B+ ewes was comparatively higher than that of BB and ++ ewes. Further, it is interesting to note that heterozygous and homozygous state of individuals increased 0.70 and 1.14 extra lambs as compared to non-carriers (++), respectively.
本研究旨在评估FecB基因分型对导入FecB基因的加罗×马尔普拉（GM）杂交绵羊体重、平均日增重（ADG）、母羊生产效率（EPE）和产羔数的影响。总共随机选择了235只GM羔羊，采用强制限制性片段长度多态性聚合酶链反应（RFLP-PCR）筛选FecB突变。大多数（69.8%）GM个体是FecB突变的携带者（BB和B+），FecB等位基因频率约为0.40。FecB基因分型与羔羊出生至12月龄的体重显著相关（P<0.01）。按世代（F1、F2和F3）来看，羔羊出生时、6月龄和12月龄的体重无显著差异，但在3月龄和9月龄时差异显著（P<0.05）。不同基因型之间，ADG1（0至3个月）显著相关（P<0.05），但ADG2（3至6个月）和ADG3（6至12个月）并非如此。出生类型和性别对出生至12月龄的体重有显著影响（P<0.01）；单羔羔羊从出生至12月龄的体重显著高于双羔和三羔羔羊（P<0.01）。出生类型对ADG1有显著影响（P<0.01），但对ADG2和ADG3无显著影响。出生年份不影响出生体重和断奶体重，但对断奶后各年龄段的体重和ADG有显著影响（P<0.01）。FecB基因分型在出生时、3月龄和12月龄时对EPE有显著影响（P<0.01）。在12月龄时，B+和BB母羊的EPE分别比++母羊高7.86千克（36.9%）和2.32千克（10.9%）。BB母羊的平均产羔数（2.17±0.24）显著高于B+母羊（1.73±0.04）和++母羊（1.03±0.23）（P<0.01）。本研究表明，携带突变基因的羔羊（BB和B+）的体重和ADG相对低于非携带者（++），而B+母羊的EPE相对高于BB和++母羊。此外，值得注意的是，与非携带者（++）相比，杂合子和纯合子个体分别多产0.70只和1.14只羔羊。
