Jurado-Campos Alejandro, Soria-Meneses Pedro Javier, Arenas-Moreira María, Alonso-Moreno Carlos, Rodríguez-Robledo Virginia, Soler Ana Josefa, Garde José Julián, Del Rocío Fernández-Santos María
SaBio IREC (CSIC-UCLM-JCCM), Campus Universitario, S/N, 02071, Albacete, Spain.
Departamento de Química Inorgánica, Orgánica Y Bioquímica-Centro de Innovación en Química Avanzada (ORFEO-CINQA), Facultad de Farmacia, Universidad de Castilla-La Mancha, 02008, Albacete, Spain.
J Anim Sci Biotechnol. 2023 Aug 10;14(1):106. doi: 10.1186/s40104-023-00907-3.
Artificial insemination (AI) is a routine breeding technology in animal reproduction. Nevertheless, the temperature-sensitive nature and short fertile lifespan of ram sperm samples hamper its use in AI. In this sense, nanotechnology is an interesting tool to improve sperm protection due to the development of nanomaterials for AI, which could be used as delivery vehicles. In this work, we explored the feasibility of vitamin E nanoemulsion (NE) for improving sperm quality during transport.
With the aim of evaluating this proposal, ejaculates of 7 mature rams of Manchega breed were collected by artificial vagina and extended to 60 × 10 spz/mL in Andromed®. Samples containing control and NE (12 mmol/L) with and without exogenous oxidative stress (100 µmol/L Fe/ascorbate) were stored at 22 and 15 ºC and motility (CASA), viability (YO-PRO/PI), acrosomal integrity (PNA-FITC/PI), mitochondrial membrane potential (Mitotracker Deep Red 633), lipoperoxidation (C BODIPY 581/591), intracellular reactive oxygen species (ROS) production and DNA status (SCSA®) monitored during 96 h. Our results show that NE could be used to maintain ram spermatozoa during transport at 15 and 22 ºC for up to 96 h, with no appreciable loss of kinematic and physiological characteristics of freshly collected samples.
The storage of ram spermatozoa in liquid form for 2-5 d with vitamin E nanoemulsions may lead more flexibility to breeders in AI programs. In view of the potential and high versatility of these nanodevices, further studies are being carried out to assess the proposed sperm preservation medium on fertility after artificial insemination.
人工授精（AI）是动物繁殖中的一项常规育种技术。然而，公羊精子样本对温度敏感的特性以及较短的可育寿命阻碍了其在人工授精中的应用。从这个意义上说，由于用于人工授精的纳米材料的发展，纳米技术是一种改善精子保护的有趣工具，这些纳米材料可用作递送载体。在这项工作中，我们探索了维生素E纳米乳液（NE）在运输过程中改善精子质量的可行性。
为了评估这一方案，通过人工阴道收集了7只曼彻格品种成年公羊的精液，并在Andromed®中稀释至60×10精子/mL。含有对照和NE（12 mmol/L）且有或无外源性氧化应激（100 μmol/L铁/抗坏血酸盐）的样本在22℃和15℃下储存，并在96小时内监测活力（CASA）、存活率（YO-PRO/PI）、顶体完整性（PNA-FITC/PI）、线粒体膜电位（Mitotracker Deep Red 633）、脂质过氧化（C BODIPY 581/591）、细胞内活性氧（ROS）产生和DNA状态（SCSA®）。我们的结果表明，NE可用于在15℃和22℃下运输期间保存公羊精子长达96小时，新鲜采集样本的运动学和生理学特征没有明显损失。
用维生素E纳米乳液将公羊精子以液体形式储存2 - 5天可能会给人工授精项目中的育种者带来更大的灵活性。鉴于这些纳米器件的潜力和高度通用性，正在进行进一步的研究，以评估所提议的精子保存介质对人工授精后生育能力的影响。
