• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

脱氧胆酸对食管腺癌侵袭的影响：对基质金属蛋白酶的影响。

Impact of Deoxycholic Acid on Oesophageal Adenocarcinoma Invasion: Effect on Matrix Metalloproteinases.

机构信息

School of Pharmacy and Pharmaceutical Sciences, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2 D02, Ireland.

School of Medicine, Trinity Translational Medicine Institute, Trinity College Dublin, Trinity Centre for Health Sciences, St James's Hospital, Dublin 8 D08, Ireland.

出版信息

Int J Mol Sci. 2020 Oct 28;21(21):8042. doi: 10.3390/ijms21218042.

DOI:10.3390/ijms21218042
PMID:33126685
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7672620/
Abstract

Bile acids (BAs) have been implicated in the development of oesophagitis, Barrett's oesophagus and oesophageal adenocarcinoma (OAC). However, whether BAs promote cancer invasiveness has not been elucidated. We evaluated the role of BAs, in particular deoxycholic acid (DCA), in OAC invasion. Migration and invasiveness in untreated and BA-treated oesophageal SKGT-4 cancer cells were evaluated. Activity and expression of different matrix metalloproteinases (MMPs) were determined by zymography, ELISA, PCR and Western blot. Finally, human OAC tissues were stained for MMP-10 by immunohistochemistry. It was found that SKGT-4 cells incubated with low concentrations of DCA had a significant increase in invasion. In addition, MMP-10 mRNA and protein expression were also increased in the presence of DCA. MMP-10 was found to be highly expressed both in-vitro and in-vivo in neoplastic OAC cells relative to non-neoplastic squamous epithelial cells. Our results show that DCA promotes OAC invasion and MMP-10 overexpression. This study will advance our understanding of the pathophysiological mechanisms involved in human OAC and shows promise for the development of new therapeutic strategies.

摘要

胆汁酸（BAs）与食管炎、巴雷特食管和食管腺癌（OAC）的发展有关。然而，BAs 是否促进癌症侵袭性尚未阐明。我们评估了 BAs，特别是脱氧胆酸（DCA）在 OAC 侵袭中的作用。未处理和 BA 处理的食管 SKGT-4 癌细胞的迁移和侵袭性进行了评估。通过酶谱分析、ELISA、PCR 和 Western blot 测定不同基质金属蛋白酶（MMPs）的活性和表达。最后，通过免疫组织化学法检测 MMP-10 在人 OAC 组织中的染色。结果发现，DCA 孵育的 SKGT-4 细胞侵袭明显增加。此外，DCA 存在时 MMP-10 的 mRNA 和蛋白表达也增加。与非肿瘤性鳞状上皮细胞相比，MMP-10 在体外和体内的肿瘤性 OAC 细胞中均高度表达。我们的研究结果表明，DCA 促进 OAC 的侵袭和 MMP-10 的过表达。这项研究将增进我们对涉及人类 OAC 的病理生理机制的理解，并为开发新的治疗策略提供了希望。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1794/7672620/5719ed3b858f/ijms-21-08042-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1794/7672620/2ccea67938d6/ijms-21-08042-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1794/7672620/716966dc387d/ijms-21-08042-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1794/7672620/957236a9dcc3/ijms-21-08042-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1794/7672620/f1b3e216a499/ijms-21-08042-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1794/7672620/80cceda6c740/ijms-21-08042-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1794/7672620/5719ed3b858f/ijms-21-08042-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1794/7672620/2ccea67938d6/ijms-21-08042-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1794/7672620/716966dc387d/ijms-21-08042-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1794/7672620/957236a9dcc3/ijms-21-08042-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1794/7672620/f1b3e216a499/ijms-21-08042-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1794/7672620/80cceda6c740/ijms-21-08042-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1794/7672620/5719ed3b858f/ijms-21-08042-g006.jpg

相似文献

1
Impact of Deoxycholic Acid on Oesophageal Adenocarcinoma Invasion: Effect on Matrix Metalloproteinases.脱氧胆酸对食管腺癌侵袭的影响：对基质金属蛋白酶的影响。
Int J Mol Sci. 2020 Oct 28;21(21):8042. doi: 10.3390/ijms21218042.
2
Opposing effects of bile acids deoxycholic acid and ursodeoxycholic acid on signal transduction pathways in oesophageal cancer cells.胆汁酸脱氧胆酸和熊去氧胆酸对食管癌细胞信号转导通路的相反作用。
Eur J Cancer Prev. 2016 Sep;25(5):368-79. doi: 10.1097/CEJ.0000000000000198.
3
Bile acid-induced expression of activation-induced cytidine deaminase during the development of Barrett's oesophageal adenocarcinoma.胆酸诱导激活诱导胞苷脱氨酶在巴雷特食管腺癌发展过程中的表达。
Carcinogenesis. 2011 Nov;32(11):1706-12. doi: 10.1093/carcin/bgr194. Epub 2011 Sep 1.
4
Increased expression of matrix metalloproteinases mediates thromboxane A2-induced invasion in lung cancer cells.基质金属蛋白酶表达增加介导血栓素 A2 诱导的肺癌细胞侵袭。
Curr Cancer Drug Targets. 2012 Jul;12(6):703-15. doi: 10.2174/156800912801784884.
5
An integrative genomic approach in oesophageal cells identifies TRB3 as a bile acid responsive gene, downregulated in Barrett's oesophagus, which regulates NF-kappaB activation and cytokine levels.食管细胞的综合基因组方法确定 TRB3 为一种胆汁酸反应基因，在巴雷特食管中下调，其调节 NF-κB 激活和细胞因子水平。
Carcinogenesis. 2010 May;31(5):936-45. doi: 10.1093/carcin/bgq036. Epub 2010 Feb 5.
6
Investigating the pathogenic role of PADI4 in oesophageal cancer.研究 PADI4 在食管癌中的致病作用。
Int J Biol Sci. 2011;7(6):769-81. doi: 10.7150/ijbs.7.769. Epub 2011 Jun 11.
7
Upregulation of sine oculis homeobox homolog 3 is associated with proliferation, invasion, migration, as well as poor prognosis of esophageal cancer.Sine oculis homeobox homolog 3 的上调与食管癌的增殖、侵袭、迁移以及不良预后有关。
Anticancer Drugs. 2019 Jul;30(6):596-603. doi: 10.1097/CAD.0000000000000751.
8
The bile acid deoxycholic acid has a non-linear dose response for DNA damage and possibly NF-kappaB activation in oesophageal cells, with a mechanism of action involving ROS.胆汁酸脱氧胆酸对食管细胞中的DNA损伤以及可能的核因子-κB激活具有非线性剂量反应，其作用机制涉及活性氧。
Mutagenesis. 2008 Sep;23(5):399-405. doi: 10.1093/mutage/gen029. Epub 2008 May 30.
9
Expression of neuroepithelial transforming gene 1 is enhanced in oesophageal cancer and mediates an invasive tumour cell phenotype.神经上皮转化基因 1 的表达在食管癌中增强，并介导侵袭性肿瘤细胞表型。
J Exp Clin Cancer Res. 2013 Aug 14;32(1):55. doi: 10.1186/1756-9966-32-55.
10
Dietary inflammatory index and risk of reflux oesophagitis, Barrett's oesophagus and oesophageal adenocarcinoma: a population-based case-control study.饮食炎症指数与反流性食管炎、巴雷特食管和食管腺癌风险：一项基于人群的病例对照研究。
Br J Nutr. 2017 May;117(9):1323-1331. doi: 10.1017/S0007114517001131. Epub 2017 Jun 2.

引用本文的文献

1
Tetrahydroxylated bile acids prevents malignant progression of Barret esophagus by inhibiting the interleukin-1β-nuclear factor kappa-B pathway.四羟基化胆汁酸通过抑制白细胞介素-1β-核因子κB通路来预防巴雷特食管的恶性进展。
World J Gastroenterol. 2025 Aug 7;31(29):107066. doi: 10.3748/wjg.v31.i29.107066.
2
Matrix Dynamics and Microbiome Crosstalk: Matrix Metalloproteinases as Key Players in Disease and Therapy.基质动力学与微生物组相互作用：基质金属蛋白酶在疾病与治疗中的关键作用
Int J Mol Sci. 2025 Apr 11;26(8):3621. doi: 10.3390/ijms26083621.
3
Bile's Hidden Weapon: Modulating the Microbiome and Tumor Microenvironment.

本文引用的文献

1
Silencing microRNA-330-5p increases MMP1 expression and promotes an invasive phenotype in oesophageal adenocarcinoma.沉默 microRNA-330-5p 可增加 MMP1 表达并促进食管腺癌的侵袭表型。
BMC Cancer. 2019 Aug 7;19(1):784. doi: 10.1186/s12885-019-5996-3.
2
Glyco-conjugated bile acids drive the initial metaplastic gland formation from multi-layered glands through crypt-fission in a murine model.糖缀合胆汁酸通过隐窝裂变驱动多层腺体向初始化生腺形成，在小鼠模型中。
PLoS One. 2019 Jul 26;14(7):e0220050. doi: 10.1371/journal.pone.0220050. eCollection 2019.
3
Matrix metalloproteinase (MMP)-7 in Barrett's esophagus and esophageal adenocarcinoma: expression, metabolism, and functional significance.
胆汁的隐藏武器：调节微生物组和肿瘤微环境。
Curr Microbiol. 2024 Nov 30;82(1):25. doi: 10.1007/s00284-024-04004-0.
4
Definition of a Multi-Omics Signature for Esophageal Adenocarcinoma Prognosis Prediction.用于预测食管腺癌预后的多组学特征的定义
Cancers (Basel). 2024 Aug 1;16(15):2748. doi: 10.3390/cancers16152748.
5
Reflux conditions induce E-cadherin cleavage and EMT via APE1 redox function in oesophageal adenocarcinoma.反流条件通过食管腺癌中的 APE1 氧化还原功能诱导 E-钙黏蛋白裂解和 EMT。
Gut. 2023 Dec 7;73(1):47-62. doi: 10.1136/gutjnl-2023-329455.
6
The pro- and antineoplastic effects of deoxycholic acid in pancreatic adenocarcinoma cell models.脱氧胆酸在胰腺腺癌细胞模型中的促癌和抗癌作用。
Mol Biol Rep. 2023 Jun;50(6):5273-5282. doi: 10.1007/s11033-023-08453-x. Epub 2023 May 5.
7
Research Progress of Bile Acids in Cancer.胆汁酸在癌症中的研究进展
Front Oncol. 2022 Jan 20;11:778258. doi: 10.3389/fonc.2021.778258. eCollection 2021.
8
Carnosine Protects against Cerebral Ischemic Injury by Inhibiting Matrix-Metalloproteinases.肌肽通过抑制基质金属蛋白酶对脑缺血损伤起保护作用。
Int J Mol Sci. 2021 Jul 13;22(14):7495. doi: 10.3390/ijms22147495.
基质金属蛋白酶（MMP）-7在巴雷特食管和食管腺癌中的表达、代谢及功能意义
Physiol Rep. 2018 May;6(10):e13683. doi: 10.14814/phy2.13683.
4
Matrix Metalloproteinase Inhibitors in Cancer Therapy: Turning Past Failures Into Future Successes.基质金属蛋白酶抑制剂在癌症治疗中的应用：从过去的失败中汲取教训，迈向未来的成功。
Mol Cancer Ther. 2018 Jun;17(6):1147-1155. doi: 10.1158/1535-7163.MCT-17-0646. Epub 2018 May 7.
5
From Heartburn to Barrett's Esophagus, and Beyond.从胃灼热到巴雷特食管，以及其他情况。
World J Surg. 2017 Jul;41(7):1698-1704. doi: 10.1007/s00268-017-3957-z.
6
Interleukin-17A promotes esophageal adenocarcinoma cell invasiveness through ROS-dependent, NF-κB-mediated MMP-2/9 activation.白细胞介素-17A通过活性氧依赖性、核因子κB介导的基质金属蛋白酶-2/9激活促进食管腺癌细胞侵袭。
Oncol Rep. 2017 Mar;37(3):1779-1785. doi: 10.3892/or.2017.5426. Epub 2017 Feb 7.
7
Inhibition of matrix metalloproteinase-9 by a barbiturate-nitrate hybrid ameliorates dextran sulphate sodium-induced colitis: effect on inflammation-related genes.巴比妥酸盐 - 硝酸盐杂合物对基质金属蛋白酶 - 9的抑制作用可改善葡聚糖硫酸钠诱导的结肠炎：对炎症相关基因的影响
Br J Pharmacol. 2017 Apr;174(7):512-524. doi: 10.1111/bph.13712. Epub 2017 Feb 24.
8
Bile acids but not acidic acids induce Barrett's esophagus.胆汁酸而非酸性酸会诱发巴雷特食管。
Int J Clin Exp Pathol. 2015 Feb 1;8(2):1384-92. eCollection 2015.
9
Mechanisms of platelet-stimulated colon cancer invasion: role of clusterin and thrombospondin 1 in regulation of the P38MAPK-MMP-9 pathway.血小板刺激结肠癌细胞侵袭的机制：簇蛋白和血小板反应蛋白 1 在调节 P38MAPK-MMP-9 通路中的作用。
Carcinogenesis. 2014 Feb;35(2):324-32. doi: 10.1093/carcin/bgt332. Epub 2013 Oct 1.
10
In oesophageal squamous cells exposed to acidic bile salt medium, omeprazole inhibits IL-8 expression through effects on nuclear factor-κB and activator protein-1.在食管鳞状细胞暴露于酸性胆盐培养基中时，奥美拉唑通过对核因子-κB 和激活蛋白-1 的作用抑制 IL-8 的表达。
Gut. 2014 Jul;63(7):1042-52. doi: 10.1136/gutjnl-2013-305533. Epub 2013 Sep 18.