Li Yujun, Wei Shuang, Zhang Kaibo, Fang Yong, Liu Hailiang, Jin Zhanfeng, Guo Qingxia, He Jun, Song Wuqi, Zhang Fengmin
Wu Lien-Teh Institute, Department of Microbiology, Harbin Medical University, 194 Xuefu Road, Harbin, 150086, China.
Heilongjiang Provincial Key Laboratory of Infection and Immunity, Pathogen Biology, Harbin, China.
Lasers Med Sci. 2021 Sep;36(7):1411-1419. doi: 10.1007/s10103-020-03172-2. Epub 2020 Oct 31.
Because of a large number of macrophages and its secreted pro-inflammatory factors in the synovial fluid of patients with rheumatoid arthritis, the present study aimed to investigate the effect and mechanism of 630-nm LED exposure on monocytes/macrophages and its anti-inflammatory effect. The THP-1 monocytes and PMA-induced THP-1 macrophages (THP-1 macrophages) were employed and irradiated by 630-nm LED for different time and times, and then measure the pro-inflammatory cytokines production by RT-qPCR and Milliplex MAP Multiplex assay, the proteins involved in inflammation pathway and reactive oxygen species (ROS) levels in the cells were detected by Western blot and DCFH-DA method. The exposure dose of red LED (15.3 J/cm, 30.6 J/cm) were determined as no-influence on the cell proliferation, the pro-inflammatory factors TNF-α and IL-1β mRNAs, and secretions in supernatant of THP-1 macrophages were significantly decreased after LED exposure. The ROS production was blocked in THP-1 monocytes and THP-1 macrophages after treatment of LED. Finally, the phosphorylated NF-κB proteins which involved in inflammation pathway significantly decreased, and its inhibitors Nrf2 were slightly upregulated. The effects of LED anti-inflammation response are dependent on the mechanism of inhibiting ROS level and regulating NF-κB signaling pathways by increasing Nrf2 expression in the cells. It is suggested that 630-nm LED could decrease pro-inflammation in immune cells, and it may be a beneficial adjunct therapy in relieving inflammation of patients with rheumatoid arthritis.
由于类风湿性关节炎患者滑液中存在大量巨噬细胞及其分泌的促炎因子，本研究旨在探讨630纳米发光二极管（LED）照射对单核细胞/巨噬细胞的影响及机制及其抗炎作用。采用THP-1单核细胞和经佛波酯（PMA）诱导的THP-1巨噬细胞（THP-1巨噬细胞），用630纳米LED进行不同时间和次数的照射，然后通过逆转录定量聚合酶链反应（RT-qPCR）和多重免疫检测法检测促炎细胞因子的产生，用蛋白质免疫印迹法和2',7'-二氯二氢荧光素二乙酸酯（DCFH-DA）法检测细胞中炎症通路相关蛋白和活性氧（ROS）水平。确定红色LED的照射剂量（15.3 J/cm²、30.6 J/cm²）对细胞增殖无影响，LED照射后THP-1巨噬细胞上清液中的促炎因子肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）的信使核糖核酸（mRNAs）及分泌量显著降低。LED处理后，THP-1单核细胞和THP-1巨噬细胞中的ROS产生被阻断。最后，参与炎症通路的磷酸化核因子-κB（NF-κB）蛋白显著减少，其抑制剂核因子E2相关因子2（Nrf2）略有上调。LED抗炎反应的作用依赖于通过增加细胞中Nrf2表达来抑制ROS水平和调节NF-κB信号通路的机制。提示630纳米LED可降低免疫细胞中的促炎作用，可能是缓解类风湿性关节炎患者炎症的一种有益辅助治疗方法。
