Gaber Mona, Galal Lamia A A, Hassan Dalia, Badary Dalia M, Mohamed Israa M A, Elossily Nahed
Department of Parasitology, Faculty of Medicine, Assiut University, Major General Ahmed Kamel Street, Al-Qasr Road, Assiut 71515, Egypt.
Department of Animal Hygiene, Faculty of Veterinary Medicine, Assiut University, Major General Ahmed Kamel Street, Al-Qasr Road, Assiut 71515, Egypt.
Ann Parasitol. 2020;66(3):311-318. doi: 10.17420/ap6603.269.
Cryptosporidium spp. is an obligate intracellular parasite that has become a community threat. The pathological consequences of cryptosporidiosis vary not only in different Cryptosporidium species but even among different isolates of the same species. The present study aimed to track the serological and immunohistopathological differences between animals infected by Cryptosporidium parvum "Iowa isolate" (CPI) and Cryptosporidium parvum "water isolate" isolated from a local water supply in Assiut Governorate, Egypt (CPW). Three experimental groups were encountered: negative control group (C), the CPI group and the CPW group; each contains ten Swiss albino mice. Serum cytokine: IL10 and TNF-α were measured. Expression of Cryptosporidium antigen and CD3 in the intestinal, pulmonary and brain tissue were evaluated through immunohistochemical assay. IL10 and TNF-α were elevated in both infected groups, over expression of Cryptosporidium protein and CD3 in the intestinal, pulmonary and brain tissue in CPW infected group compared to Iowa infected one. Multi-organs affection occurred in the CPW indicating more severe pathogenicity and virulence than standard Iowa isolate. The local C. parvum isolate was more virulent than tested Iowa isolate as it spread extra-intestinally to reach brain tissue.
隐孢子虫属是一种专性细胞内寄生虫，已成为一种公共威胁。隐孢子虫病的病理后果不仅因不同的隐孢子虫种类而异，甚至在同一物种的不同分离株之间也有所不同。本研究旨在追踪感染微小隐孢子虫“爱荷华分离株”（CPI）和从埃及阿斯尤特省当地供水系统分离出的微小隐孢子虫“水分离株”（CPW）的动物之间的血清学和免疫组织病理学差异。设立了三个实验组：阴性对照组（C）、CPI组和CPW组；每组包含十只瑞士白化小鼠。检测血清细胞因子白细胞介素10（IL10）和肿瘤坏死因子-α（TNF-α）。通过免疫组织化学分析评估肠道、肺和脑组织中隐孢子虫抗原和CD3的表达。两个感染组中的IL10和TNF-α均升高，与感染爱荷华分离株的组相比，CPW感染组的肠道、肺和脑组织中隐孢子虫蛋白和CD3表达过高。CPW组出现多器官感染，表明其致病性和毒力比标准爱荷华分离株更强。当地的微小隐孢子虫分离株比测试的爱荷华分离株毒性更强，因为它会通过肠道外扩散到达脑组织。
