Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, 50 Ngam Wong Wan Rd, Ladyaow, Chatuchak, Bangkok 10900, Thailand.
Department of Forest Product, Faculty of Forestry, Kasetsart University, 50 Ngam Wong Wan Rd, Ladyaow, Chatuchak, Bangkok 10900, Thailand.
Bioresour Technol. 2021 Jan;320(Pt A):124298. doi: 10.1016/j.biortech.2020.124298. Epub 2020 Oct 22.
Oil palm (Elaeis guineensis) trunk chips were processed by steam explosion under different steam conditions, followed by alkaline extraction and fermentation to produce efficient lignocellulosic ethanol as sustainable alternative energy resource. The optimum condition of steam explosion was attained at 210°C for 4 min (α-cellulose: 58.83% and lignin: 27.12%). Taguchi 3 factor design [(sodium hydroxide concentration (NaOH), temperature and time)] was performed to optimize alkaline extraction. The optimum condition at 15% NaOH, 90°C for 60 min gave highest percentage α-cellulose: 87.14% and lowest percentage of lignin: 6.13%. Simultaneous saccharification and fermentation (SSF) involved 10% dry weight pretreated fibers, Celluclast 1.5L (15 FPU /gram substrate), Novozyme 188 (15 IU/gram substrate) and Saccharomyces cerevisiae SC90. The highest ethanol concentration (C) produced during SSF was 44.25 g/L. Nonetheless, pre-hydrolysis simultaneous saccharification and fermentation gave 31.22 g/L (C). All results suggested that optimized two step pretreatment produced efficient ethanol.
油棕（Elaeis guineensis）树干碎片经过不同蒸汽条件下的蒸汽爆破处理，然后进行碱性提取和发酵，以生产高效的木质纤维素乙醇作为可持续的替代能源。蒸汽爆破的最佳条件是在 210°C 下处理 4 分钟（α-纤维素：58.83%，木质素：27.12%）。采用田口 3 因子设计[（氢氧化钠浓度（NaOH）、温度和时间）]优化碱性提取条件。在 15%NaOH、90°C 下处理 60 分钟，得到的α-纤维素最高（87.14%），木质素最低（6.13%）。同步糖化和发酵（SSF）涉及 10%干重预处理纤维、Celluclast 1.5L（每克基质 15 FPU）、Novozyme 188（每克基质 15IU）和酿酒酵母 SC90。SSF 过程中产生的最高乙醇浓度（C）为 44.25g/L。然而，预水解同步糖化和发酵可产生 31.22g/L（C）。所有结果表明，优化的两步预处理可生产高效的乙醇。
