Hari Krishna S, Chowdary G V
Biotechnology Division, Department of Chemical Engineering, Andhra University, Visakhapatnam 530 003, India.
J Agric Food Chem. 2000 May;48(5):1971-6. doi: 10.1021/jf991296z.
Simultaneous saccharification and fermentation (SSF) of alkaline hydrogen peroxide pretreated Antigonum leptopus (Linn) leaves to ethanol was optimized using cellulase from Trichoderma reesei QM-9414 (Celluclast from Novo) and Saccharomyces cerevisiae NRRL-Y-132 cells. Response surface methodology (RSM) and a three-level four-variable design were employed to evaluate the effects of SSF process variables such as cellulase concentration (20-100 FPU/g of substrate), substrate concentration (5-15% w/v), incubation time (24-72 h), and temperature (35-45 degrees C) on ethanol production efficiency. Cellulase and substrate concentrations were found to be the most significant variables. The optimum conditions arrived at are as follows: cellulase = 100 FPU/g of substrate, substrate = 15% (w/v), incubation time = 57.2 h, and temperature = 38.5 degrees C. At these conditions, the predicted ethanol yield was 3.02% (w/v) and the actual experimental value was 3.0% (w/v).
采用里氏木霉QM-9414（诺维信公司的Celluclast）的纤维素酶和酿酒酵母NRRL-Y-132细胞，对经碱性过氧化氢预处理的戟叶鹅绒藤叶片同步糖化发酵（SSF）生产乙醇的工艺进行了优化。采用响应面法（RSM）和三水平四变量设计，评估纤维素酶浓度（20 - 100 FPU/g底物）、底物浓度（5 - 15% w/v）、培养时间（24 - 72 h）和温度（35 - 45℃）等SSF工艺变量对乙醇生产效率的影响。结果发现纤维素酶和底物浓度是最显著的变量。得到的最佳条件如下：纤维素酶 = 100 FPU/g底物，底物 = 15%（w/v），培养时间 = 57.2 h，温度 = 38.5℃。在这些条件下，预测的乙醇产率为3.02%（w/v），实际实验值为3.0%（w/v）。
