Laboratory of Veterinary Public Health, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan.
Laboratory of Veterinary Food Hygiene, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan.
Comp Immunol Microbiol Infect Dis. 2020 Dec;73:101570. doi: 10.1016/j.cimid.2020.101570. Epub 2020 Oct 25.
We examined Bartonella prevalence in 281 bat flies collected from 114 eastern bent-wing bats (Miniopterus fuliginosus) in Japan and phylogenetically analyzed with other bat fly and bat strains. The bat flies were identified as Penicilidia jenynsii (PJ; n = 45), Nycteribia allotopa (NA; n = 157), and novel Nycteribia species (NS; n = 79). Bartonella DNAs were detected in 31.7 % (89/281) of bat flies by PCR targeting the citrate synthase (gltA) gene. The prevalence of Bartonella DNA among the bat flies was 47.1 % (74/157) in NA, 15.2 % (12/79) in NS, and 6.7 % (3/45) in PJ. Bartonella bacteria were also isolated from two NA and one NS. A phylogenetic analysis of the gltA sequences revealed that bat fly-associated strains were classified into three lineages and the same lineages of Bartonella were commonly detected from both Nycteribia bat flies and Miniopterus bats. These results suggest that Nycteribia bat flies are potential vectors for transmitting Bartonella among Miniopterus bats.
我们从日本 114 只东部弯翅蝙蝠（Miniopterus fuliginosus）中采集了 281 只蝙蝠蝇，并对其进行了巴氏杆菌属流行情况检查，同时与其他蝙蝠蝇和蝙蝠株系进行了系统发育分析。这些蝙蝠蝇被鉴定为 Penicilidia jenynsii（PJ；n = 45）、Nycteribia allotopa（NA；n = 157）和新型 Nycteribia 物种（NS；n = 79）。通过针对柠檬酸合酶（gltA）基因的 PCR 检测到 31.7%（89/281）的蝙蝠蝇中存在巴氏杆菌 DNA。在 NA 中，巴氏杆菌 DNA 的流行率为 47.1%（74/157），在 NS 中为 15.2%（12/79），在 PJ 中为 6.7%（3/45）。从 2 只 NA 和 1 只 NS 中也分离出了巴氏杆菌。gltA 序列的系统发育分析显示，蝙蝠蝇相关菌株分为三个谱系，并且从 Nycteribia 蝙蝠蝇和 Miniopterus 蝙蝠中都共同检测到了相同的巴氏杆菌谱系。这些结果表明，Nycteribia 蝙蝠蝇可能是在 Miniopterus 蝙蝠中传播巴氏杆菌的潜在媒介。
