Department of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Department of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Peptides. 2021 Jan;135:170433. doi: 10.1016/j.peptides.2020.170433. Epub 2020 Oct 29.
Central kisspeptin action is well known in reproductive regulation; however, its peripheral action is not well understood. This study aimed to 1) compare serum or cerebrospinal fluid (CSF) kisspeptin levels between different body mass index (BMI) groups 2) compare the levels of kisspeptin between serum and CSF, and 3) determine correlations between serum or CSF kisspeptin levels with clinical, metabolic, and reproductive parameters. There were 40 male subjects undergoing operations with lumbar puncture anesthesia. Subgroup analysis was performed to compare between the normal (n = 12), overweight (n = 10), and obese groups (n = 17). One lean subject was recruited for correlation analysis. Serum kisspeptin levels were significantly higher in the obese group when compared to the normal weight and overweight groups even after adjusting for age or diastolic blood pressure (DBP) (p < 0.05 all). Serum leptin levels were significantly higher in the obese group when compared to the normal weight and overweight groups (p < 0.05 all). CSF kisspeptin levels were below the minimum detectable concentration for the assay (<0.06 ng/mL). Serum kisspeptin was positively correlated with body weight, BMI, plasma insulin, the homeostatic model assessment for insulin resistance (HOMA-IR), and serum leptin but was negatively correlated with plasma LH (p < 0.05 all). In conclusion, serum kisspeptin was related to obesity, leptin, insulin, and insulin resistance, while CSF kisspeptin was below the limits of detection. Thus, peripheral kisspeptin might have a role in metabolic regulation.
中央 kisspeptin 的作用在生殖调节中广为人知；然而，其外周作用尚未被充分理解。本研究旨在：1）比较不同身体质量指数（BMI）组之间的血清或脑脊液（CSF）kisspeptin 水平；2）比较血清和 CSF 中的 kisspeptin 水平；3）确定血清或 CSF kisspeptin 水平与临床、代谢和生殖参数之间的相关性。有 40 名男性受试者在腰椎穿刺麻醉下接受手术。进行亚组分析以比较正常组（n = 12）、超重组（n = 10）和肥胖组（n = 17）之间的差异。招募了一名瘦受试者进行相关性分析。即使在校正年龄或舒张压（DBP）后，与正常体重和超重组相比，肥胖组的血清 kisspeptin 水平明显更高（p < 0.05 均）。与正常体重和超重组相比，肥胖组的血清瘦素水平明显更高（p < 0.05 均）。CSF kisspeptin 水平低于检测限（<0.06 ng/mL）。血清 kisspeptin 与体重、BMI、血浆胰岛素、胰岛素抵抗稳态模型评估（HOMA-IR）和血清瘦素呈正相关，但与血浆 LH 呈负相关（p < 0.05 均）。总之，血清 kisspeptin 与肥胖、瘦素、胰岛素和胰岛素抵抗有关，而 CSF kisspeptin 低于检测限。因此，外周 kisspeptin 可能在代谢调节中发挥作用。
