Könsgen Dominique, Klinkmann Gerd, Kaul Anne, Diesing Karoline, Sehouli Jalid, Braicu Ioana, Sümnig Ariane, Erb Holger H H, Stope Matthias B, Mustea Alexander
Department of Gynecology and Gynecological Oncology, University Hospital Bonn, Venusberg-Campus 1, 53127 Bonn, Germany.
Department of Anesthesiology and Intensive Care Medicine, University Medical Center Rostock, Schillingallee 35, 18057 Rostock, Germany.
Eur J Obstet Gynecol Reprod Biol. 2020 Dec;255:154-159. doi: 10.1016/j.ejogrb.2020.10.044. Epub 2020 Oct 21.
Ovarian cancer (OC) is the leading cause of death in gynecological oncology, primarily caused by limited prognostic and therapeutic options. The heat shock protein 27 (HSP27) is recognized as a prominent factor in OC, playing a pivotal role in cancer progression machinery such as treatment resistance. Thus, HSP27 may represent an appropriate biomarker for OC diagnosis, prognosis, and therapy response.
MATERIALS & METHODS: Extracellular HSP27 levels were measured by enzyme-linked immunosorbent assay (ELISA) in serum samples of OC patients (n = 242) and compared to a non-malignant control group without any history of cancer (n = 200). Correlations between serum levels of HSP27 and clinical pathological parameters were analyzed by bivariate analysis. Survival analyses were carried out by Kaplan-Meier test.
This study demonstrated that protein levels of HSP27 are comparable in the blood serum of healthy women and OC patients. However, HSP27 levels are significantly correlated with the volume of ascites, residual tumor mass, and age at first diagnosis in OC patients. Notably, elevated levels of HSP27 demonstrate significantly higher overall survival.
Taken together, our findings demonstrate that high levels of circulating HSP27 in serum are associated with improved overall survival of OC patients. Even though functionality of secreted HSP27 is still unclear, serum levels of HSP27 represent a putative non-invasive prognostic biomarker candidate for OC progression.
卵巢癌（OC）是妇科肿瘤死亡的主要原因，主要是由于预后和治疗选择有限。热休克蛋白27（HSP27）被认为是OC中的一个重要因素，在癌症进展机制如治疗抵抗中起关键作用。因此，HSP27可能是OC诊断、预后和治疗反应的合适生物标志物。
采用酶联免疫吸附测定（ELISA）法检测OC患者（n = 242）血清样本中细胞外HSP27水平，并与无癌症病史的非恶性对照组（n = 200）进行比较。采用双变量分析血清HSP27水平与临床病理参数之间的相关性。采用Kaplan-Meier检验进行生存分析。
本研究表明，健康女性和OC患者血清中HSP27的蛋白水平相当。然而，HSP27水平与OC患者的腹水量、残余肿瘤质量和首次诊断时的年龄显著相关。值得注意的是，HSP27水平升高表明总生存期显著延长。
综上所述，我们的研究结果表明，血清中循环HSP27水平高与OC患者总生存期改善相关。尽管分泌型HSP27的功能仍不清楚，但HSP27血清水平代表了OC进展的一种假定的非侵入性预后生物标志物候选物。
