Dash Biplab, Sahu Narayan, Singh Anup Kumar, Gupta S B, Soni Ravindra
Department of Agricultural Microbiology, College of Agriculture, Indira Gandhi Krishi Vishwavidyalaya, Krishak Nagar Jora, Raipur, 492012, CG, India.
Folia Microbiol (Praha). 2021 Apr;66(2):189-196. doi: 10.1007/s12223-020-00832-2. Epub 2020 Oct 31.
In the present study, bacterial isolates were screened for arsenic resistance efficiency. Environmental isolates were isolated from arsenic-rich soil samples (i.e., from Rajnandgaon district of Chhattisgarh state, India). Amplification and sequencing of 16S rRNA gene revealed that the isolates were of Bacillus firmus RSN1, Brevibacterium senegalense RSN2, Enterobacter cloacae RSN3, Stenotrophomonas pavanii RSN6, Achromobacter mucicolens RSN7, and Ochrobactrum intermedium RSN10. Arsenite efflux gene (arsB) was successfully amplified in E. cloacae RSN3. Atomic absorption spectroscopy (AAS) analysis showed an absorption of 32.22% arsenic by the RSN3 strain. Furthermore, results of scanning electron microscopy (SEM) for morphological variations revealed an initial increase in the cell size at 1 mM sodium arsenate; however, it was decreased at 10 mM concentration in comparison to control. This change of the cell size in different metal concentrations was due to the uptake and expulsion of the metal from the cell, which also confirmed the arsenite efflux system.
在本研究中，对细菌分离株进行了抗砷效率筛选。环境分离株从富含砷的土壤样本（即来自印度恰蒂斯加尔邦拉金德冈地区）中分离得到。16S rRNA基因的扩增和测序表明，这些分离株分别为坚强芽孢杆菌RSN1、塞内加尔短杆菌RSN2、阴沟肠杆菌RSN3、帕氏寡养单胞菌RSN6、黏液无色杆菌RSN7和中间苍白杆菌RSN10。在阴沟肠杆菌RSN3中成功扩增出亚砷酸盐外排基因（arsB）。原子吸收光谱（AAS）分析显示，RSN3菌株对砷的吸收量为32.22%。此外，扫描电子显微镜（SEM）对形态变化的分析结果表明，在1 mM砷酸钠作用下细胞大小最初增加；然而，与对照相比，在10 mM浓度下细胞大小减小。不同金属浓度下细胞大小的这种变化是由于细胞对金属的摄取和排出，这也证实了亚砷酸盐外排系统。
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