Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 50931, Cologne, Germany.
Institute of Genetics, Biological Research Centre, Szeged, H-6701, P.O.Box 521, Hungary.
Dev Biol. 2021 Jan 1;469:135-143. doi: 10.1016/j.ydbio.2020.10.006. Epub 2020 Oct 23.
Transdifferentiation is a conversion of an already differentiated cell type into another cell type without the involvement of stem cells. This transition is well described in the case of vertebrate immune cells, as well as in Drosophila melanogaster, which therefore serves as a suitable model to study the process in detail. In the Drosophila larva, the latest single-cell sequencing methods enabled the clusterization of the phagocytic blood cells, the plasmatocytes, which are capable of transdifferentiation into encapsulating cells, the lamellocytes. Here we summarize the available data of the past years on the plasmatocyte-lamellocyte transition, and make an attempt to harmonize them with transcriptome-based blood cell clustering to better understand the underlying mechanisms of transdifferentiation in Drosophila, and in general.
转分化是指一种已经分化的细胞类型在不涉及干细胞的情况下转化为另一种细胞类型。这种转变在脊椎动物免疫细胞以及黑腹果蝇中得到了很好的描述，因此果蝇成为研究该过程的合适模型。在果蝇幼虫中，最新的单细胞测序方法使吞噬血细胞——浆血细胞能够聚类，浆血细胞能够转分化为包被细胞——表皮细胞。在这里，我们总结了过去几年关于浆血细胞-表皮细胞转化的可用数据，并尝试将其与基于转录组的血细胞聚类相协调，以更好地理解果蝇中转分化的潜在机制，以及一般的转分化机制。
