希腊爱奥尼亚群岛的佛手柑的植物化学特征：一种强调新橙皮苷类黄酮分布的多分析方法。

Phytochemical characteristics of bergamot oranges from the Ionian islands of Greece: A multi-analytical approach with emphasis in the distribution of neohesperidose flavanones.

机构信息

Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, 15771 Athens, Greece.

出版信息

Food Chem. 2021 May 1;343:128400. doi: 10.1016/j.foodchem.2020.128400. Epub 2020 Oct 15.

DOI:10.1016/j.foodchem.2020.128400

PMID:33131954

Abstract

The present study describes the peculiar phytochemical characteristics of bergamots cultivated in distinct islands of the Ionian Sea. Ultrahigh-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS) supported by 1 and 2D NMR spectroscopy was used for unambiguous metabolic profiling of albedo, flavedo and juice samples. Profile differences were determined using a multi-analytical clustering approach based on high-performance thin-layer chromatography fingerprints and UHPLC-HRMS data. Finally, a validated HPLC method offering good precision (0.12-0.94%) and accuracy (95.25-103.93%) was proposed for the quantification of the major flavanones. A total of 37 secondary metabolites were characterized belonging to flavonoids, limonoids and coumarins. Their distribution was tissue-dependent and varied significantly from bergamots of other geographical locations. Surprisingly, neoeriocitrin was the major flavanone, reaching 1.69 ± 0.05 g/L in the juice and 5.24 ± 0.12 mg/g in albedo. This is the highest reported amount among Citrus species, rendering Ionian bergamots a promising candidate for novel functional products.

摘要

本研究描述了在爱奥尼亚海不同岛屿种植的佛手柑的特殊植物化学特征。采用超高效液相色谱-高分辨率质谱联用（UHPLC-HRMS）结合 1D 和 2D NMR 光谱法，对桔皮、桔络和果汁样品进行了明确的代谢轮廓分析。使用基于高效薄层色谱指纹图谱和 UHPLC-HRMS 数据的多分析聚类方法确定了图谱差异。最后，提出了一种经验证的 HPLC 方法，用于定量测定主要黄烷酮，该方法具有良好的精密度（0.12-0.94%）和准确度（95.25-103.93%）。共鉴定出 37 种次生代谢产物，属于类黄酮、柠檬苦素和香豆素。它们的分布与组织有关，与其他地理位置的佛手柑差异显著。令人惊讶的是，新橙皮苷是主要的黄烷酮，在果汁中达到 1.69±0.05 g/L，在桔皮中达到 5.24±0.12 mg/g。这是在柑橘属植物中报道的最高含量，使爱奥尼亚佛手柑成为有前途的新型功能性产品的候选者。