测定选定物种中的类黄酮及其抗胶原酶和抗氧化潜力。

Determination of Flavonoids in Selected Species and Their Anti-Collagenase and Antioxidant Potential.

机构信息

Department of Pharmacognosy, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, ul. Mickiewicza 2a, 15-230 Białystok, Poland.

Microbiota Lab, Center for Preclinical Studies, Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy, Medical University of Warsaw, ul. Banacha 1, 02-097 Warsaw, Poland.

出版信息

Molecules. 2022 Mar 21;27(6):2015. doi: 10.3390/molecules27062015.

DOI:10.3390/molecules27062015

PMID:35335375

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8951040/

Abstract

A new 5,7-dihydroxy-3'-methoxy-4'-acetoxyflavone-8---d-arabinopyranoside-2″--(4‴-acetoxy)-glucoside () and three known flavone -glycosides-5,7,3',4'-tetrahydroxyflavone-6--xyloside-8---d-glucoside (lucenin-1) (), 5,7,3'-trihydroxyflavone-6--glucoside-8---d-glucoside (vicenin-2) (), and 5,7,4'-trihydroxy-3'-methoxyflavone-6---d-glucopyranoside-8---arabinopyranoside (chrysoeriol-6---d-glucopyranoside-8---arabinopyranoside) ()-were isolated from aerial parts of L. (Caryophyllaceae). Their structures were determined through the use of comprehensive spectroscopic and spectrometric methods, and a method for the quantification of the major constituents of and L. was developed. Furthermore, the anti-collagenase and antioxidant activities of all isolated compounds obtained from extracts and fractions from both species were evaluated. The highest percentage of collagenase inhibition (at 400 µg/mL) was distinguished for methanolic extracts (22.06%, 32.04%) and ethyl acetate fractions (16.59%, 14.40%) from and . Compounds - displayed moderate inhibitory activity, with IC values ranging from 39.59-73.86 µM.

摘要

一种新的 5,7-二羟基-3'-甲氧基-4'-乙酰氧基黄酮-8---D-阿拉伯吡喃糖苷-2″--(4‴-乙酰氧基)-葡萄糖苷 () 和三种已知的黄酮苷-5,7,3',4'-四羟基黄酮-6--木糖苷-8---D-葡萄糖苷 (卢塞宁-1) (), 5,7,3'-三羟基黄酮-6--葡萄糖苷-8---D-葡萄糖苷 (vicenin-2) (), 和 5,7,4'-三羟基-3'-甲氧基黄酮-6---D-葡萄糖吡喃糖苷-8---阿拉伯吡喃糖苷 (chrysoeriol-6---D-葡萄糖吡喃糖苷-8---阿拉伯吡喃糖苷) ()-从 L. 的地上部分分离得到 (石竹科)。通过使用综合光谱和光谱方法确定了它们的结构，并建立了一种定量测定和 L.主要成分的方法。此外，还评估了从这两种植物的提取物和馏分中获得的所有分离化合物的抗胶原酶和抗氧化活性。在 400 µg/mL 时，来自和的甲醇提取物（22.06%、32.04%）和乙酸乙酯馏分（16.59%、14.40%）显示出最高的胶原酶抑制率。化合物 - 显示出中等抑制活性，IC 值范围为 39.59-73.86 µM。