Terada Takumi, Nii Takahiro, Isobe Naoki, Yoshimura Yukinori
Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.
Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.
J Poult Sci. 2020 Oct 25;57(4):310-318. doi: 10.2141/jpsa.0190098.
The aim of this study was to determine the effects of live probiotics (LR) and (CB) on the expression of genes of innate immune system in broiler chick ileum and cecum. Chicks were administered 500 water with or without LR or CB, daily from day 1 to 6 after hatching. The ileum and cecum were collected on day 7 for analysis of gene expression of Toll-like receptors (TLRs), pro- and anti-inflammatory cytokines, and antimicrobial peptides (AMPs) using real-time PCR. The expression of was upregulated by CB in the ileum and that of was upregulated by both LR and CB. Expression of and in the ileum and of and in the cecum was upregulated by both LR and CB. The gene expressions of avian -defensin () and cathelicidin () were upregulated by CB and that of was upregulated by LR in the cecum. However, the expression of in the ileum was downregulated by LR. These results suggest that probiotic LR and CB treatments affect a part of the innate defense system in the ileum and cecum by modulating the expression of innate immune molecules including TLRs, pro- and anti-inflammatory cytokines, and AMPs.
本研究的目的是确定活性益生菌（LR）和（CB）对肉仔鸡回肠和盲肠中固有免疫系统基因表达的影响。从孵化后第1天到第6天，每天给雏鸡饮用含有或不含有LR或CB的500水。在第7天收集回肠和盲肠，使用实时PCR分析Toll样受体（TLR）、促炎和抗炎细胞因子以及抗菌肽（AMP）的基因表达。CB使回肠中的表达上调，LR和CB均使的表达上调。LR和CB均使回肠中的和以及盲肠中的和的基因表达上调。CB使盲肠中的禽β-防御素（）和cathelicidin（）的基因表达上调，LR使盲肠中的的表达上调。然而，LR使回肠中的表达下调。这些结果表明，益生菌LR和CB处理通过调节包括TLR、促炎和抗炎细胞因子以及AMP在内的固有免疫分子的表达，影响回肠和盲肠中的部分固有防御系统。
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