Nii Takahiro, Jirapat Jirapat, Isobe Naoki, Yoshimura Yukinori
Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan.
Research Center for Animal Science, Hiroshima University, Higashi-Hiroshima, Japan.
J Poult Sci. 2020 Jan 25;57(1):67-76. doi: 10.2141/jpsa.0190035.
Probiotic bacteria are known for their beneficial effects on the intestinal immune function of the host animal. However, their effects on mucosal barrier function in chicks are not completely understood. The aim of this study was to determine the effects of the probiotic bacterium, (LR), on the gastrointestinal mucosal barrier function of broiler chicks. One day-old male broiler chicks were orally injected water (300 L) with or without 1 × 10 cfu of LR (5 mg FINELACT, Asahi Calpis Wellness Co. Ltd.) every morning for 7 days (day 0 to 6). The crop, duodenum, ileum, and cecum were collected on day 7 and were used for histological analysis and RNA extraction. Then, the thickness of the mucosal structures and the number of goblet cells in the digestive tract were assessed using histological analysis. The expression of Mucin 2, factors related to the formation of tight junctions (Claudin1, 5, and 16, ZO2, and JAM2), cytokines (IL-6, CXCLi2, and IL-10), and avian -defensin 10 (AvBDs) (AvBD2, 10, and 12) in the crop, duodenum, ileum, and cecum were analyzed using real-time polymerase chain reaction (PCR). Results showed that oral administration of LR increased ileal villus height and crypt depth, decreased Claudin16 level in the crop and increased JAM2 level in the crop and ileum, and decreased the expression of AvBD10 in the ileum and cecum and that of AvBD12 in the crop. It did not affect goblet cell number and Mucin 2 expression. These results suggested that LR used in this study may enhance mucosal barrier function by regulating tight junctions in the upper gastrointestinal tract.
益生菌对宿主动物的肠道免疫功能具有有益作用，这是众所周知的。然而，它们对雏鸡黏膜屏障功能的影响尚未完全明确。本研究旨在确定益生菌（LR）对肉鸡胃肠道黏膜屏障功能的影响。1日龄雄性肉鸡雏鸡每天早晨口服300微升水，其中一组添加1×10⁸cfu的LR（Finelact 5毫克，朝日可尔必思健康有限公司），另一组不添加，持续7天（第0天至第6天）。在第7天采集嗉囊、十二指肠、回肠和盲肠，用于组织学分析和RNA提取。然后，通过组织学分析评估消化道黏膜结构的厚度和杯状细胞的数量。使用实时聚合酶链反应（PCR）分析嗉囊、十二指肠、回肠和盲肠中黏蛋白2、紧密连接形成相关因子（Claudin1、5和16、ZO2和JAM2）、细胞因子（IL-6、CXCLi2和IL-10）以及禽β-防御素10（AvBDs）（AvBD2、10和12）的表达。结果表明，口服LR可增加回肠绒毛高度和隐窝深度，降低嗉囊中Claudin16水平，增加嗉囊和回肠中JAM2水平，并降低回肠和盲肠中AvBD10以及嗉囊中AvBD12的表达。它对杯状细胞数量和黏蛋白2表达没有影响。这些结果表明，本研究中使用的LR可能通过调节上消化道的紧密连接来增强黏膜屏障功能。
