Zhao Yaqiong, Cheng Pei, Zhang Yuzhu, Wang Haiguang
Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, China.
Front Microbiol. 2020 Oct 2;11:542961. doi: 10.3389/fmicb.2020.542961. eCollection 2020.
The emergence of new physiological races of f. sp. () causing wheat stripe rust can lead to the loss of resistance of wheat cultivars to stripe rust, thus resulting in severe losses in wheat yield. In this study, after the germination of urediospores of three strains including the original strain (CYR32, a dominant physiological race of in China) and two virulence-mutant strains (CYR32-5 and CYR32-61) acquired from CYR32 via UV-B radiation, proteomic analysis based on isobaric tags for relative and absolute quantification (iTRAQ) technology was performed on the strains. A total of 2,271 proteins were identified, and 59, 74, and 64 differentially expressed proteins (DEPs) were acquired in CYR32-5 vs. CYR32, CYR32-61 vs. CYR32, and CYR32-61 vs. CYR32-5, respectively. The acquired DEPs were mainly involved in energy metabolism, carbon metabolism, and cellular substance synthesis. Furthermore, quantitative reverse transcription PCR assays were used to determine the relative expression of the 6, 7, and 1 DEPs of CYR32-5 vs. CYR32, CYR32-61 vs. CYR32, and CYR32-61 vs. CYR32-5, respectively, at the transcriptional level. The relative expression levels of one, five, and one gene, respectively, encoding the DEPs, were consistent with the corresponding protein abundance determined by iTRAQ technology. Compared with CYR32, the DEPs associated with energy metabolism and stress-including E3JWK6, F4S0Z3, and A8N2Q4-were up-regulated in the mutant strains. The results indicated that the virulence-mutant strains CYR32-5 and CYR32-61 had more tolerance to stress than the original strain CYR32. The results obtained in this study are of great significance for exploring the virulence variation mechanisms of , monitoring the changes in populations, breeding new disease-resistant wheat cultivars, and managing wheat stripe rust sustainably.
引起小麦条锈病的小麦条锈菌新生理小种的出现，会导致小麦品种对条锈病的抗性丧失，从而造成小麦产量的严重损失。在本研究中，包括原始菌株（CYR32，中国条锈菌的一个优势生理小种）以及通过紫外线B辐射从CYR32获得的两个毒性突变菌株（CYR32 - 5和CYR32 - 61）在内的三个菌株的夏孢子萌发后，对这些菌株进行了基于相对和绝对定量等压标签（iTRAQ）技术的蛋白质组分析。共鉴定出2271种蛋白质，在CYR32 - 5与CYR32、CYR32 - 61与CYR32以及CYR32 - 61与CYR32 - 5的比较中，分别获得了59、74和64个差异表达蛋白（DEP）。获得的DEP主要参与能量代谢、碳代谢和细胞物质合成。此外，采用定量逆转录PCR分析分别测定了CYR32 - 5与CYR32、CYR32 - 61与CYR32以及CYR32 - 61与CYR32 - 5中6、7和1个DEP在转录水平上的相对表达。分别编码DEP的一个、五个和一个基因的相对表达水平与通过iTRAQ技术测定的相应蛋白质丰度一致。与CYR32相比，与能量代谢和应激相关的DEP（包括E3JWK6、F4S0Z3和A8N2Q4）在突变菌株中上调。结果表明，毒性突变菌株CYR32 - 5和CYR32 - 61比原始菌株CYR32对胁迫具有更强的耐受性。本研究获得的结果对于探索条锈菌的毒性变异机制、监测条锈菌群体变化、培育新的抗病小麦品种以及可持续管理小麦条锈病具有重要意义。
