Jin Min, Chen Yu-Lei, He Xiongfei, Hou Yanping, Chan Zhuhua, Zeng Runying
Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, China.
Southern Marine Science and Engineering Guangdong Laboratory, Zhuhai, China.
Front Microbiol. 2020 Sep 29;11:567060. doi: 10.3389/fmicb.2020.567060. eCollection 2020.
Androgenetic alopecia (AGA) is a dihydrotestosterone (DHT)-mediated hair loss disorder characterized by shortened anagen hair cycle. Oligosaccharides derived from seaweeds possess diverse biological functions. However, little is known about their effects on AGA. In this study, algal oligosaccharide (AOS) was characterized for its mitigation effects on key features involved in AGA pathogenesis, such as DHT- mediated cellular signaling and shortened anagen hair cycle. AOS with varying degrees of polymerization (DP), namely, AOS (DP2), AOS (DP4-6), and AOS (DP8-12), were prepared by agar biodegradation with WPAGA1, an agarolytic bacterium isolated from deep-sea sediments. results showed that AOS with varying DPs significantly ameliorated the DHT-induced alterations of regulatory factors in human hair follicle dermal papilla cells in a dose- and DP-dependent manner, as revealed by the normalization of several hair-growth-stimulating or inhibitory factors. studies showed that AOS (DP2) extended the anagen phase and thereby delayed catagen progression in mice. Furthermore, AOS (DP2) stimulated dorsal hair growth in mice by increasing hair length, density, and thickness. Therefore, our findings indicated that AOS antagonized key factors involved in AGA pathogenesis, suggesting the potential application of AOS in the prevention and the treatment of AGA.
雄激素性脱发（AGA）是一种由二氢睾酮（DHT）介导的脱发疾病，其特征是生长期毛发周期缩短。源自海藻的寡糖具有多种生物学功能。然而，关于它们对AGA的影响知之甚少。在本研究中，对藻寡糖（AOS）减轻AGA发病机制中关键特征（如DHT介导的细胞信号传导和缩短的生长期毛发周期）的作用进行了表征。通过用从深海沉积物中分离出的琼脂分解菌WPAGA1对琼脂进行生物降解，制备了具有不同聚合度（DP）的AOS，即AOS（DP2）、AOS（DP4 - 6）和AOS（DP8 - 12）。结果表明，不同DP的AOS以剂量和DP依赖性方式显著改善了DHT诱导的人毛囊真皮乳头细胞中调节因子的改变，这通过几种毛发生长刺激或抑制因子的正常化得以揭示。研究表明，AOS（DP2）延长了小鼠的生长期，从而延迟了退行期的进展。此外，AOS（DP2）通过增加毛发长度、密度和厚度刺激了小鼠背部毛发的生长。因此，我们的研究结果表明AOS拮抗了AGA发病机制中的关键因素，提示AOS在AGA的预防和治疗中具有潜在应用价值。
