Sadeghi Abbas, Farokhi Farah, Shalizar-Jalali Ali, Najafi Gholamreza
Department of Biology, Faculty of Science, Urmia University, Urmia, Iran.
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Vet Res Forum. 2020 Summer;11(3):257-263. doi: 10.30466/vrf.2019.91184.2206. Epub 2020 Sep 15.
Polyvinyl chloride (PVC) has toxic effects through the induction of oxidative stress in the body and testicles. Vitamin E (Vit E) is a dietary compound that functions as an antioxidant scavenging toxic free radicals. The present study aimed to probe the protective effect of Vit E against PVC-induced reprotoxicity in male rats. In this experimental study, 24 male rats were randomly divided into four groups (n=6) including control, Vit E (150 mg kg per day; orally), PVC (1000 mg kg per day; orally) and PVC + Vit E. After 40 days, rats were euthanized and epididymal sperms characteristics, embryo development and malondialdehyde (MDA) and testosterone levels were examined. The PVC decreased sperm count, motility and viability as well as testosterone level and increased sperms with damaged chromatin in comparison with controls. Also, the percentages of fertilization, two-cell embryos and blastocysts as well as MDA levels were decreased in PVC-treated rats. However, Vit E improved PVC-induced alterations in aforesaid parameters. The results indicated that PVC can reduce fertility potential in male rats probably through androgen and sperm quality and quantity reductions, while Vit E can exert protective effects in PVC-related reproductive toxicities.
聚氯乙烯（PVC）通过在体内和睾丸中诱导氧化应激而产生毒性作用。维生素E（Vit E）是一种膳食化合物，具有清除有毒自由基的抗氧化剂功能。本研究旨在探讨Vit E对PVC诱导的雄性大鼠生殖毒性的保护作用。在这项实验研究中，24只雄性大鼠被随机分为四组（n = 6），包括对照组、Vit E组（每天150毫克/千克；口服）、PVC组（每天1000毫克/千克；口服）和PVC + Vit E组。40天后，对大鼠实施安乐死，并检查附睾精子特征、胚胎发育以及丙二醛（MDA）和睾酮水平。与对照组相比，PVC降低了精子数量、活力和存活率以及睾酮水平，并增加了染色质受损的精子。此外，PVC处理的大鼠的受精率、二细胞胚胎和囊胚的百分比以及MDA水平均降低。然而，Vit E改善了PVC引起的上述参数变化。结果表明，PVC可能通过降低雄激素以及精子的质量和数量来降低雄性大鼠的生育潜力，而Vit E可以对PVC相关的生殖毒性发挥保护作用。
