Zarei Leila, Sadrkhanlou Rajabali, Shahrooz Rasoul, Malekinejad Hassan, Eilkhanizadeh Behroz, Ahmadi Abbas
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
Department of Pathology, Faculty of Medicine, University of Medical Sciences, Urmia, Iran.
Vet Res Forum. 2014 Winter;5(1):21-7.
This study was aimed to assess the protective effects of Cornus mas fruit extract (CMFE) and vitamin E (Vit E) on sperm quality parameters in the methotrexate (MTX)-treated mice. Forty-eight young adult male mice (8-12 weeks) were randomly divided into six groups including control and test groups. The control group received normal saline orally , and the test groups were treated MTX (20 mg kg(-1), ip, once weekly), MTX + CMFE (250 mg kg(-1)), MTX + CMFE (500 mg kg(-1)), MTX + CMFE (1000 mg kg(-1)), and MTX + Vit E (100 IU kg(-1), po) for 35 consecutive days. On day 35, after euthanasia the epididymal sperms were isolated. Then the total mean sperm count, sperm viability and motility were determined. The total antioxidant capacity (TAOC) of all experimental groups were also evaluated. The MTX-treated animals showed a significant changes in all parameters of sperm quality assessment compared to the control group. Both Vit E and CMFE were able to protect from MTX-induced effects on sperm maturity and DNA damage. Co-administration of MTX and CMFE and/or Vit E resulted in protection from MTX-reduced TAOC. In conclusion, these data suggested that MTX administration could adversely affect the sperm quality. Moreover, the protective effect of Vit E and CMFE on MTX-induced sperm toxicity was also documented.
本研究旨在评估山茱萸果实提取物（CMFE）和维生素E（Vit E）对甲氨蝶呤（MTX）处理的小鼠精子质量参数的保护作用。48只8 - 12周龄的成年雄性小鼠被随机分为六组，包括对照组和试验组。对照组口服生理盐水，试验组分别接受MTX（20 mg kg(-1)，腹腔注射，每周一次）、MTX + CMFE（250 mg kg(-1)）、MTX + CMFE（500 mg kg(-1)）、MTX + CMFE（1000 mg kg(-1)）以及MTX + Vit E（100 IU kg(-1)，口服）连续处理35天。在第35天，安乐死后分离附睾精子。然后测定精子总数、活力和运动能力。还评估了所有实验组的总抗氧化能力（TAOC）。与对照组相比，MTX处理的动物在精子质量评估的所有参数上均出现显著变化。Vit E和CMFE均能保护精子免受MTX诱导的成熟影响和DNA损伤。MTX与CMFE和/或Vit E联合使用可防止MTX降低TAOC。总之，这些数据表明MTX给药可能对精子质量产生不利影响。此外，还记录了Vit E和CMFE对MTX诱导的精子毒性的保护作用。
