Pagès Marielle, Kleiber Didier, Violleau Frédéric
Equipe Physiologie Pathologie et Génétique Végétales (PPGV) Institut Polytechnique de Toulouse Université de Toulouse Toulouse Cedex 03 France.
Laboratoire de Chimie Agro-industrielle (LCA) INRA, INPT, INP-EI PURPAN Université de Toulouse Toulouse France.
Food Sci Nutr. 2020 Aug 19;8(10):5292-5297. doi: 10.1002/fsn3.1618. eCollection 2020 Oct.
Although ozone (O) is a well-known bactericide and fungicide, the required dose of ozone can depend significantly on the targeted pathogens. The present research evaluates the variation in sensibility to ozone of three fungal species from a single fungal group. The three fungal species selected, , , and , belong to the Ascomycota group and attack apples. The fungi were exposed to ozone by bubbling directly into the spore solutions (treatment period ranged from 0.5 to 4 min, ozone concentration in inlet gas ranged from 1 to 30 g/m). The rates of germination were determined, and the level of peroxidation of the lipid membrane was quantified based on the malondialdehyde (MDA) production. The results indicate that ozone effectively reduces spore development and suggest that the fungi differ in sensitivity. To reduce by 50% the spore germination rate of , , and requires ozone doses of 0.01, 0.03, and 0.07 mg/ml, respectively. Spore sensitivity seems to be directly linked to spore surface. For all the fungal species, the MDA level and the level of spore inactivation both increase with ozone dose, which confirms that ozone alters the cell membrane.
尽管臭氧（O）是一种广为人知的杀菌剂和杀真菌剂，但所需的臭氧剂量可能会因目标病原体的不同而有显著差异。本研究评估了来自单一真菌类群的三种真菌对臭氧敏感性的变化。所选择的三种真菌，即 、 和 ，属于子囊菌门，会侵害苹果。通过直接向孢子溶液中鼓泡使真菌暴露于臭氧中（处理时间为0.5至4分钟，进气中的臭氧浓度为1至30克/立方米）。测定了发芽率，并基于丙二醛（MDA）的产生量对脂质膜的过氧化水平进行了量化。结果表明，臭氧能有效抑制孢子发育，且表明这些真菌的敏感性存在差异。要使 、 和 的孢子发芽率降低50%，分别需要0.01、0.03和0.07毫克/毫升的臭氧剂量。孢子敏感性似乎与孢子表面直接相关。对于所有真菌种类，MDA水平和孢子失活水平均随臭氧剂量的增加而升高，这证实了臭氧会改变细胞膜。
