Sehim Amira E, Abd Elghaffar Rasha Y, Emam Amany M, El-Desoukey Tarek A
Botany and Microbiology Department, Faculty of Science, Benha University, Benha, Egypt.
Department of Food Toxicology and Contaminant, National Research Centre, Dokki, Giza, Egypt.
Heliyon. 2023 Jul 3;9(7):e17885. doi: 10.1016/j.heliyon.2023.e17885. eCollection 2023 Jul.
Toxigenic fungi infect fruits and vegetables either during harvest or storage and create mycotoxins as secondary metabolites, which pose a serious threat to human and animal health throughout the food chain. Therefore, the objective of this study was to determine the inhibitory effect of OZO against the growth and spore germination of the fungal strain. Additionally, evaluation of the synthesis inhibition of toxins (ATs), among which are alternariol (AOH), alternariol-9-methyl ether (AME), and tenuazonic acid (TeA) in the potato dextrose broth (PDB) medium and orange fruit after harvest. The results indicated that the inhibition zone was 29.0 ± 1.2 mm at 20 mg/L of OZO. The MIC and MFC values were recorded at 0.186 and 1.57 mg/mL, respectively. In this regard, OZO prevented conidia germination at 98.8% with the treatment of 5 mg/mL. OZO at 20 mg/mL was efficacious in producing a high loss in ATs production in the PDB medium, reaching 73.4, 76, and 67.1% for AOH, AME, and TeA, respectively. In addition, OZO prevents the biosynthesis of AOH and AME during the storage of orange fruits compared with the positive control sample. In contrast, 20 mg/mL reduced TeA accumulation and the appearance of brown spot (ABS) in orange. To the best of our knowledge, this is the first report that studies OZO to control ATs in vitro in orange fruits.
产毒真菌在收获或储存期间感染水果和蔬菜，并产生霉菌毒素作为次生代谢产物，这对整个食物链中的人类和动物健康构成严重威胁。因此，本研究的目的是确定OZO对该真菌菌株生长和孢子萌发的抑制作用。此外，评估在马铃薯葡萄糖肉汤（PDB）培养基和收获后的橙子果实中，毒素（ATs）合成的抑制情况，其中包括链格孢酚（AOH）、链格孢酚-9-甲醚（AME）和细交链孢菌酮酸（TeA）。结果表明，在20mg/L的OZO浓度下，抑菌圈为29.0±1.2mm。MIC和MFC值分别记录为0.186和1.57mg/mL。在这方面，5mg/mL的OZO处理可使分生孢子萌发率降低98.8%。20mg/mL的OZO能有效降低PDB培养基中ATs的产量，其中AOH、AME和TeA的产量分别降低73.4%、76%和67.1%。此外，与阳性对照样品相比，OZO可在橙子果实储存期间抑制AOH和AME的生物合成。相比之下，20mg/mL的OZO可减少橙子中TeA的积累和褐斑（ABS）的出现。据我们所知，这是第一份研究OZO在橙子果实中体外控制ATs的报告。
