一种用于铜的比率检测的香豆素基荧光探针及其在生物成像中的应用。

A Coumarin-Based Fluorescent Probe for Ratiometric Detection of Cu and Its Application in Bioimaging.

作者信息

Zhang Jie, Chen Meng-Yu, Bai Cui-Bing, Qiao Rui, Wei Biao, Zhang Lin, Li Rui-Qian, Qu Chang-Qing

机构信息

School of Chemistry and Materials Engineering, Fuyang Normal University, Fuyang, China.

Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry of the Chinese Academy of Sciences (TIPC-CAS), Beijing, China.

出版信息

Front Chem. 2020 Oct 2;8:800. doi: 10.3389/fchem.2020.00800. eCollection 2020.

The fluorescent probe , based on naphthalimide-modified coumarin, was designed, synthesized, and characterized, which could recognize Cu from other cations selectively and sensitively in HEPES buffer (10 mM, Ph = 7. 4)/CHCN (1:4, V/V). When the probe interacted with Cu, the color and the fluorescent intensity changed obviously and it provided the naked-eye detection for Cu. The recognition mode between them was achieved by Job's plot, IR, MS, SEM, and HNMR. In addition, test strips made from could still interact with Cu in tap water effectively. The limit of detection (LOD) of was 3.5 × 10 M. Additionally, the density functional theory (DFT) calculation method was used to analyze the action mechanism of toward Cu. Importantly, the fluorescent probe could demonstrate favorable selectivity toward Cu in . Thus, was considered to have some potential for application in bioimaging.

基于萘二甲酰亚胺修饰香豆素的荧光探针被设计、合成及表征，其能够在HEPES缓冲液（10 mM，pH = 7.4）/乙腈（1:4，V/V）中选择性且灵敏地从其他阳离子中识别出铜离子。当该探针与铜离子相互作用时，颜色和荧光强度发生明显变化，实现了对铜离子的裸眼检测。它们之间的识别模式通过Job曲线、红外光谱、质谱、扫描电子显微镜和核磁共振氢谱得以确定。此外，由该探针制成的测试条在自来水中仍能与铜离子有效相互作用。该探针的检测限为3.5×10⁻⁸ M。另外，采用密度泛函理论（DFT）计算方法分析了该探针与铜离子的作用机制。重要的是，荧光探针在细胞中对铜离子表现出良好的选择性。因此，该探针被认为在生物成像方面具有一定的应用潜力。