Novel Fluorescent Probe toward Fe Based on Rhodamine 6G Derivatives and Its Bioimaging in Adult Mice, , and Plant Tissues.

A new fluorescent probe based on the rhodamine 6G platforms has been designed, synthesized, and characterized, which could recognize Fe effectively in HEPES buffer (10 mM, pH = 7.4)/CHCN (2:3, v/v). And the distinct color change and the rapid emergence of fluorescence emission at 550 nm achieved "naked eye" detection of Fe. The interaction mode between them was achieved by Job's plot, MS, SEM, and X-ray single-crystal diffraction. Importantly, the crystal structures proved that Fe could induce the rhodamine moiety transform the closed-cycle form to the open-cycle form. But it is interesting that Fe did not appear in the crystal structures. Meanwhile, the limit of detection (LOD) of to Fe was calculated to be 3.47 × 10. In addition, the RGB experiment, test papers, and silica gel plates all indicated that the probe could be used to distinguish Fe quantitatively and qualitatively on-site. Moreover, the probe has also been successfully applied to Fe image in , adult mice, and plant tissues. Thus, was considered to have some potential for application in bioimaging.