Yang Jing, Zhou Mou, Li Wendan, Lin Fang, Shan Guiqiu
Department of Clinical Laboratory, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong 510055, China.
Department of Blood Transfusion, General Hospital of Southern Theatre Command of PLA, Guangzhou 510010, China.
ACS Omega. 2020 Oct 13;5(42):27113-27118. doi: 10.1021/acsomega.0c02543. eCollection 2020 Oct 27.
The management and treatment of chronic wounds or acute wounds remain a major challenge in modern medicine. The application of autologous platelet-rich plasma (PRP) has become a promising adjuvant therapy to promote wound healing. PRP is derived from centrifuged whole blood to extract concentrated platelets, and a large amount of cytokines and growth factors are released upon activation. These bioactive molecules can enhance angiogenesis and tissue regeneration. Herein, PRP-loaded gelatin microspheres were prepared by the emulsion cross-linking method. Scanning electron microscopy results showed that the prepared microspheres are completely spherical, with an average particle size of 15.95 ± 3.79 μm and having a uniform particle size. Among them, the surface of a single microsphere is smooth and has a microporous structure, which may be the main channel for drug diffusion. Results of drug release measurements show that the prepared microspheres can slowly release the vascular endothelial growth factor for more than 7 days. cell experiments show that the prepared microspheres can promote proliferation and migration of L929 mouse fibroblast cells. In summary, the prepared PRP-loaded gelatin microspheres with high and long-term activity can provide experimental and theoretical knowledge for the development of the clinical long-acting injectable formulations.
慢性伤口或急性伤口的管理与治疗仍是现代医学中的一项重大挑战。自体富血小板血浆（PRP）的应用已成为促进伤口愈合的一种有前景的辅助治疗方法。PRP由离心全血提取浓缩血小板而来，激活后会释放大量细胞因子和生长因子。这些生物活性分子可增强血管生成和组织再生。在此，通过乳液交联法制备了负载PRP的明胶微球。扫描电子显微镜结果显示，制备的微球呈完全球形，平均粒径为15.95±3.79μm，粒径均匀。其中，单个微球表面光滑且具有微孔结构，这可能是药物扩散的主要通道。药物释放测量结果表明，制备的微球可在7天以上缓慢释放血管内皮生长因子。细胞实验表明，制备的微球可促进L929小鼠成纤维细胞的增殖和迁移。综上所述，制备的具有高活性和长效性的负载PRP的明胶微球可为临床长效注射制剂的开发提供实验和理论依据。
