Key Laboratory of Plant Genetics and Breeding, College of Agriculture, Guangxi University, Nanning, 530005, China.
Department of Agronomy, Faculty of Crop Production Sciences, The University of Agriculture, Peshawar, 25000, Pakistan.
Physiol Plant. 2021 Dec;173(4):1295-1308. doi: 10.1111/ppl.13253. Epub 2020 Nov 18.
Salinity is a potential abiotic stress and globally threatens crop productivity. However, the molecular mechanisms underlying salt stress tolerance with respect to cytoplasmic effect, gene expression, and metabolism pathway under salt stress have not yet been reported in isonuclear kenaf genotypes. To fill this knowledge gap, growth, physiological, biochemical, transcriptome, and cytoplasm changes in kenaf cytoplasmic male sterile (CMS) line (P3A) and its iso-nuclear maintainer line (P3B) under 200 mM sodium chloride (NaCl) stress and control conditions were analyzed. Salt stress significantly reduced leaf soluble protein, soluble sugars, proline, chlorophyll content, antioxidant enzymatic activity, and induced oxidative damage in terms of higher MDA contents in both genotypes. The reduction of these parameters resulted in a reduced plant growth compared with control. However, P3A was relatively more tolerant to salt stress than P3B. This tolerance of P3A was further confirmed by improved physio-biochemical traits under salt stress conditions. Transcriptome analysis showed that 4256 differentially expressed genes (DEGs) between the two genotypes under salt stress were identified. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that photosynthesis, photosynthesis antenna-protein, and plant hormone signal transduction pathways might be associated with the improved NaCl stress tolerance in P3A. Conclusively, P3A cytoplasmic male sterile could be a potential salt-tolerant material for future breeding program of kenaf and can be used for phytoremediation of salt-affected soils. These data provide a valuable resource on the cytoplasmic effect of salt-responsive genes in kenaf and salt stress tolerance in kenaf.
盐度是一种潜在的非生物胁迫因素，全球范围内对作物生产力构成威胁。然而，在同核红麻基因型中，关于细胞质效应、基因表达和代谢途径的耐盐分子机制尚未见报道。为了填补这一知识空白，本研究分析了 200mmol/L 氯化钠（NaCl）胁迫和对照条件下红麻细胞质雄性不育（CMS）系（P3A）及其同核保持系（P3B）的生长、生理、生化、转录组和细胞质变化。盐胁迫显著降低了两种基因型叶片可溶性蛋白、可溶性糖、脯氨酸、叶绿素含量、抗氧化酶活性，并导致 MDA 含量升高，引发氧化损伤。这些参数的降低导致植物生长与对照相比受到抑制。然而，P3A 对盐胁迫的耐受性相对高于 P3B。P3A 在盐胁迫条件下改善了生理生化特性，进一步证实了其对盐胁迫的耐受性。转录组分析表明，在盐胁迫下，两种基因型之间有 4256 个差异表达基因（DEGs）。京都基因与基因组百科全书（KEGG）通路分析表明，光合作用、光合作用天线蛋白和植物激素信号转导途径可能与 P3A 提高 NaCl 胁迫耐受性有关。综上所述，P3A 细胞质雄性不育系可能是未来红麻育种计划中一种有潜力的耐盐材料，并可用于盐渍土壤的植物修复。这些数据为红麻中盐响应基因的细胞质效应和红麻的耐盐性提供了有价值的资源。
