College of Agriculture, Guangxi University, Nanning, 530004, China.
Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, Guangxi Botanical Garden of Medicinal Plants, Nanning, 530023, China.
Sci Rep. 2021 Mar 5;11(1):5301. doi: 10.1038/s41598-021-84567-1.
Kenaf is a great source of bast fiber and possesses significantly industrial interests. Cytoplasmic male sterility (CMS) is the basis of heterosis utilization in kenaf. Chloroplast, an important organelle for photosynthesis, could be associated with CMS. To understand the phylogenetic position and molecular basis of kenaf CMS from the perspective of chloroplast, the chloroplast (cp) genomes of the CMS line P3A and its maintainer line P3B were characterized and their comparative analysis was also performed. In this study, the chloroplast genomes of P3B and P3A were sequenced with 163,597 bp and 163,360 bp in length, respectively. A total of 131 genes including 85 protein coding genes (PCGs), 38 transfer RNA (tRNA) genes, and 8 ribosome RNA (rRNA) genes were annotated in P3B, while 132 genes containing 83 PCGs, 41 tRNA genes, and 8 rRNA genes were found in P3A. The phylogenetic tree revealed that kenaf was closely related to Hibiscus syriacus and Abelmoschus esculentus. Further analysis of single nucleotide polymorphism (SNP) and insertion and deletion (InDel) showed that compared with P3B, a total of 22 SNPs and 53 InDels were detected in gene coding region, gene intron, and intergenic regions of P3A. Remarkably, a total of 9 SNPs including 6 synonymous SNPs and 3 nonsynonymous SNPs were found in psbK, atpA, rpoC2, atpB, rpl20, clpP, rpoA, and ycf1. The present study provided basic information for further study of kenaf CMS mechsnism.
麻疯树是一种重要的韧皮纤维来源，具有重要的工业价值。细胞质雄性不育（CMS）是麻疯树杂种优势利用的基础。叶绿体是光合作用的重要细胞器，可能与 CMS 有关。为了从叶绿体的角度了解麻疯树 CMS 的系统发育地位和分子基础，对 CMS 系 P3A 和其保持系 P3B 的叶绿体基因组进行了特征描述，并对其进行了比较分析。在本研究中，对 P3B 和 P3A 的叶绿体基因组进行了测序，长度分别为 163597bp 和 163360bp。P3B 共注释了 131 个基因，包括 85 个蛋白编码基因（PCGs）、38 个转移 RNA（tRNA）基因和 8 个核糖体 RNA（rRNA）基因，而 P3A 则发现了 132 个基因，包含 83 个 PCGs、41 个 tRNA 基因和 8 个 rRNA 基因。系统发育树表明，麻疯树与黄蜀葵和食用黄葵密切相关。进一步的单核苷酸多态性（SNP）和插入缺失（InDel）分析表明，与 P3B 相比，P3A 的基因编码区、基因内含子和基因间区共检测到 22 个 SNP 和 53 个 InDel。值得注意的是，在 psbK、atpA、rpoC2、atpB、rpl20、clpP、rpoA 和 ycf1 中发现了总共 9 个 SNP，包括 6 个同义 SNP 和 3 个非同义 SNP。本研究为进一步研究麻疯树 CMS 机制提供了基础信息。
