Marine Science and Technology College, Zhejiang Ocean University, Zhoushan 316021, China.
College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China.
Genes (Basel). 2023 Jul 13;14(7):1440. doi: 10.3390/genes14071440.
The black sea bass, , is a potential candidate for commercial aquaculture. Due to inadequate removal of nitrogen in its breeding environment, exhibits increased nitrate concentration, which can cause acute toxicity, including energy metabolism damage and tissue damage. Therefore, RNA-seq technology was applied to characterize genes associated with toxicity tolerance under nitrate stress. The nitrate treatment caused significant changes in a total of 8920 genes, of which 2949 genes were up-regulated and 5971 genes were down-regulated. It was found that significantly enriched GO terms and KEGG were associated with blood microparticles, inhibitors of enzyme activity, and complement and coagulation cascade pathways. Furthermore, through bioinformatics analysis, it was found that these different pathways obtained in GO and KEGG enrichment analysis were mostly related to the immune and inflammatory response of fish. This study expands our understanding of the mechanism of nitrate stress affecting the liver function of .
黑鲈，是一种有商业养殖潜力的鱼类。由于其繁殖环境中氮的去除不充分，会导致硝酸盐浓度增加，从而引起急性毒性，包括能量代谢损伤和组织损伤。因此，本研究采用 RNA-seq 技术来表征硝酸盐胁迫下与毒性耐受相关的基因。硝酸盐处理导致总共 8920 个基因发生显著变化，其中 2949 个基因上调，5971 个基因下调。结果发现，显著富集的 GO 术语和 KEGG 与血液微粒体、酶活性抑制剂以及补体和凝血级联途径相关。此外，通过生物信息学分析，发现 GO 和 KEGG 富集分析中获得的这些不同途径主要与鱼类的免疫和炎症反应有关。本研究扩展了我们对硝酸盐胁迫影响黑鲈肝脏功能的机制的理解。
