Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina - UFSC, Florianópolis, SC, Brazil.
Laboratory of Agroecology, Federal University of Fronteira Sul - UFFS, Erechim, Brazil.
Biotechnol Appl Biochem. 2021 Dec;68(6):1469-1478. doi: 10.1002/bab.2067. Epub 2020 Nov 10.
The use of enzymatic catalysts is an alternative to chemical catalysts as they can help to obtain products with less environmental impact, considered sustainable within the concept of green chemistry. The optimization, kinetic, lipase reuse, and scale-up of enzymatic production of ethylene glycol oleate in the batch mode were carried out using the NS 88011 lipase in a solvent-free system. For the optimization step, a 2 Central Composite Design was used and the optimized condition for the ethylene glycol oleate production, with conversions above 99%, was at 70 °C, 600 rpm, substrates molar ratio of 1:2, 1 wt% of NS 88011 in 32 H of reaction. Kinetic tests were also carried out with different amounts of enzyme, and it showed that by decreasing the amount of the enzyme, the conversion also decreases. The lipase reuse showed good conversions until the second cycle of use, after which it had a progressive reduction reaching 83% in the fourth cycle of use. The scale-up (ninefold increase) showed promising results, with conversion above 99%, achieving conversions similar to small-scale reactions. Therefore, this work proposed an environmentally safe route to produce an emollient ester using a low-cost biocatalyst in a solvent-free system.
酶催化剂的使用是化学催化剂的替代方法，因为它们可以帮助获得对环境影响较小的产品，在绿色化学的概念中被认为是可持续的。在无溶剂体系中使用 NS 88011 脂肪酶，对批次模式下油酸乙二醇酯的酶法生产进行了优化、动力学、脂肪酶重复使用和放大。在优化步骤中，使用了 2 中心组合设计，油酸乙二醇酯生产的优化条件是在 70°C、600rpm、底物摩尔比为 1:2、反应 32 小时下 NS 88011 的浓度为 1wt%，转化率超过 99%。还进行了不同酶量的动力学测试，结果表明，随着酶量的减少，转化率也降低。脂肪酶重复使用显示出良好的转化率，直到第二次使用循环，之后转化率逐渐降低，第四次使用循环时达到 83%。放大（增加九倍）显示出有希望的结果，转化率超过 99%，达到与小规模反应相似的转化率。因此，这项工作提出了一种使用低成本生物催化剂在无溶剂体系中生产滋润酯的环保安全方法。
