Drug Repurposing and Translational Research Lab, Institute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organization , Delhi, India.
Aqua Research Laboratory, Department of Zoology, University of Delhi , Delhi, India.
Cell Adh Migr. 2020 Dec;14(1):214-226. doi: 10.1080/19336918.2020.1842131.
For successful transplantation of Hematopoietic Stem cells (HSCs), it is quite necessary that efficient homing, engraftment and retention of HSC self-renewal capacity takes place, which is often restricted due to inadequate number of adult HSCs. Here, we report that short-term treatment of mouse bone marrow mononuclear cells (BMMNCs) to Sodium Hydrogen Sulfide (NaHS, hydrogen sulfide-HS donor) can be used as a possible strategy to overcome such hurdle. HS increases the expression of CXCR4 on HSPCs, enhancing their migration toward SDF-1α in-vitro and thus homing to BM niche. . Additionally, studies revealed that HS has a role in activating mitochondria, thus, pushing quiescent HSCs into division. These results suggest a readily available and cost-effective method to facilitate efficient HSC transplantation.
为了成功移植造血干细胞（HSCs），高效的归巢、植入和维持 HSC 的自我更新能力是非常必要的，但由于成人 HSCs 的数量不足，这往往受到限制。在这里，我们报告说，短期治疗小鼠骨髓单核细胞（BMMNCs）的硫化氢（NaHS，硫化氢-HS 供体）可以作为一种克服这一障碍的可能策略。HS 增加了 HSPCs 上 CXCR4 的表达，增强了它们向 SDF-1α 的体外迁移，从而归巢到 BM 龛位。此外，研究表明 HS 在线粒体激活中起作用，从而推动静止的 HSCs 进入分裂。这些结果表明了一种现成且具有成本效益的方法，可以促进高效的 HSC 移植。
