Department of Prosthodontics, Center of Stomatology, Xiangya Hospital, Central South University, Changsha, PR China.
Department of Anesthesiology, Anesthesia Research Institute, The Second Xiangya Hospital, Central South University, Changsha, PR China.
J Biomater Appl. 2021 Mar;35(8):1061-1070. doi: 10.1177/0885328220969558. Epub 2020 Nov 1.
Three types of Co-xZr (x = 5, 7.5, and 10 wt.%) were treated with hydroxyapatite (HA) and used as an object to investigate the effect of HA coating on the surface and biocompatibility of Co-xZr alloys. And the protein adsorption and the subsequent biological behaviour of osteoblast, fibroblast and macrophages were also investigated. The surface microstructure and wettability were assessed by scanning electron microscopy (SEM) and static angle profilometer. To evaluate the biocompatibility of Co-xZr and Co-xZr-HA, we quantified plasma proteins adsorption by bicinchoninic acid assay (BCA), cytotoxicity and cell proliferation by cell counting kit-8 (CCK-8) and scanning electron microscopy (SEM). The results indicated that Co-xZr-HA alloy surfaces were more hydrophilic and had higher affinity to plasma proteins. Higher protein concentrations were found adsorbed onto Co-7.5Zr-HA and Co-10Zr-HA alloys. Cytotoxicity analysis indicated that HA coating improved the biocompatibility of Co-xZr alloys. Furthermore, the comparable results of co-incubation of Co-xZr-HA alloys with cells reveal cellular attachments to HA surfaces. HA was successfully formed on Co-xZr alloys and modified the surface structure and biocompatibility of the alloys. Co-10Zr-HA and Co-7.5Zr-HA had the most favourable properties and cytocompatibility, and therefore can be potentially used for dental implants.
三种 Co-xZr（x=5、7.5 和 10wt.%）经过羟基磷灰石（HA）处理，用作研究 HA 涂层对 Co-xZr 合金表面和生物相容性影响的对象。还研究了蛋白质吸附和随后成骨细胞、成纤维细胞和巨噬细胞的生物学行为。通过扫描电子显微镜（SEM）和静态角度轮廓仪评估表面微观结构和润湿性。为了评估 Co-xZr 和 Co-xZr-HA 的生物相容性，我们通过双缩脲法（BCA）定量测定血浆蛋白吸附，通过细胞计数试剂盒-8（CCK-8）和扫描电子显微镜（SEM）测定细胞毒性和细胞增殖。结果表明，Co-xZr-HA 合金表面具有更好的亲水性和更高的血浆蛋白亲和力。在 Co-7.5Zr-HA 和 Co-10Zr-HA 合金上发现了更高浓度的吸附蛋白质。细胞毒性分析表明，HA 涂层提高了 Co-xZr 合金的生物相容性。此外，Co-xZr-HA 合金与细胞共孵育的可比结果表明细胞附着在 HA 表面上。HA 成功地形成在 Co-xZr 合金上，修饰了合金的表面结构和生物相容性。Co-10Zr-HA 和 Co-7.5Zr-HA 具有最有利的性能和细胞相容性，因此可潜在地用于牙科植入物。
