Department of Entomology, Penn State University, University Park, PA.
J Insect Sci. 2020 Nov 1;20(6). doi: 10.1093/jisesa/ieaa027.
Deer keds (Diptera: Hippoboscidae: Lipoptena Nitzsch, 1818 and Neolipoptena Bequaert, 1942) are hematophagous ectoparasites of cervids that occasionally bite other mammals, including humans. In recent years, a number of arthropod-borne pathogens have been sequenced from deer keds. However, it is unclear if the pathogens are just present in host blood in the gut or if the pathogens are present in other organs (e.g., salivary glands) that would suggest that keds are competent vectors. Like other hippoboscoid flies, deer keds have extensive salivary glands that extend through the thorax and into the abdomen, so simply disarticulating and sequencing the thorax and abdomen separately does not circumvent the issues surrounding whole-body sequencing. Herein, we describe a technique for dissecting the terminal portion of the salivary glands from the abdomen in order to screen the thorax and salivary glands separately from the abdomen for arthropod-borne pathogens.
鹿虻（双翅目：狂蝇科：Lipoptena Nitzsch，1818 和 Neolipoptena Bequaert，1942）是鹿类的血食性外寄生虫，偶尔也会叮咬其他哺乳动物，包括人类。近年来，从鹿虻身上测序了许多节肢动物传播的病原体。然而，目前尚不清楚这些病原体是否仅存在于宿主肠道中的血液中，还是存在于其他器官（例如唾液腺）中，这表明虻是有能力的媒介。与其他狂蝇科蝇一样，鹿虻有广泛的唾液腺，延伸穿过胸部并进入腹部，因此简单地将胸部和腹部分开进行解剖和测序并不能解决全身测序所面临的问题。在此，我们描述了一种从腹部解剖唾液腺末端部分的技术，以便从腹部中分别筛选胸部和唾液腺，以寻找节肢动物传播的病原体。
