Department of Otorhinolaryngology Head and Neck Surgery, İstanbul Medeniyet University, Goztepe Training and Research Hospital, İstanbul, Turkey.
Department of Otorhinolaryngology Head and Neck Surgery, Sakarya University Training and Research Hospital, Sakarya, Turkey.
J Int Adv Otol. 2020 Dec;16(3):346-352. doi: 10.5152/iao.2020.7761.
The objective of this study was to investigate the potential protective effect of Nigella sativa oil (NSO) against cis-diamminedichloroplatinum or cisplatin (CDDP)-induced ototoxicity.
Twenty-four Wistar albino rats were randomly and equally divided into four groups. Groups 1 and 2 were given a total of 15 mg/kg CDDP intraperitoneally, which was divided equally into three doses on days 1, 3, and 5. Group 2 was treated via gavage feeding with 15 ml NSO that was divided into five doses on days 1, 3, 5, 7, and 9. Groups 3 and 4 received only 15 ml of NSO and 15 ml of 0.9% saline solution, respectively, which were orally administered and divided into five doses on days 1, 3, 5, 7, and 9. Baseline high-frequency (8, 12, 16, and 32 kHz) auditory brainstem response (ABR) measurements were collected in all the groups before the medical administrations and were repeated on the 14th day before sacrifice. Afterward, a histopathological evaluation of the cochlea was performed.
There was a significant difference in the histopathological changes between group 1 and the other groups (p<0.01). Changes in the spiral ganglion cells, the stria vascularis, and the external ciliated cells were significantly different between groups 1 and 2 (p=0.019, 0.039, and 0.045, respectively). The ABR results revealed significant differences in the 16 and 32 kHz measurements between groups 1 and 2 (p=0.013 and p<0.01, respectively).
According to the results, NSO may have a protective effect on cochlear function against the disruptive effects of CDDP in rats.
本研究旨在探讨黑种草籽油（Nigella sativa oil，NSO）对顺铂（cis-diamminedichloroplatinum 或 cisplatin，CDDP）诱导的耳毒性的潜在保护作用。
将 24 只 Wistar 白化大鼠随机均分为四组。第 1 组和第 2 组腹腔内给予总量为 15mg/kg 的 CDDP，分为 3 次剂量，分别在第 1、3 和 5 天给予。第 2 组通过灌胃给予 15ml NSO，分为 5 次剂量，分别在第 1、3、5、7 和 9 天给予。第 3 组和第 4 组分别仅给予 15ml NSO 和 15ml 0.9%生理盐水溶液，通过口服给予，分为 5 次剂量，分别在第 1、3、5、7 和 9 天给予。所有组在药物治疗前采集基线高频（8、12、16 和 32kHz）听性脑干反应（ABR）测量值，并在处死前第 14 天重复测量。之后，对耳蜗进行组织病理学评估。
第 1 组与其他组之间的组织病理学变化有显著差异（p<0.01）。第 1 组和第 2 组之间的螺旋神经节细胞、血管纹和外毛细胞的变化有显著差异（p=0.019、0.039 和 0.045）。ABR 结果显示，第 1 组和第 2 组之间在 16 和 32kHz 测量值有显著差异（p=0.013 和 p<0.01）。
根据结果，NSO 可能对耳蜗功能具有保护作用，可抵抗 CDDP 对大鼠的破坏作用。
