Department of Biochemistry, Molecular Biology, and Biophysics, Saint Paul, MN, United States of America.
Biotechnology Institute, University of Minnesota, Saint Paul, MN, United States of America.
PLoS Genet. 2020 Nov 2;16(11):e1009180. doi: 10.1371/journal.pgen.1009180. eCollection 2020 Nov.
The field performance of Sterile Insect Technique (SIT) is improved by sex-sorting and releasing only sterile males. This can be accomplished by resource-intensive separation of males from females by morphology. Alternatively, sex-ratio biasing genetic constructs can be used to selectively remove one sex without the need for manual or automated sorting, but the resulting genetically engineered (GE) control agents would be subject to additional governmental regulation. Here we describe and demonstrate a genetic method for the batch production of non-GE males. This method could be applied to generate the heterogametic sex (XY, or WZ) in any organism with chromosomal sex determination. We observed up to 100% sex-selection with batch cultures of more than 103 individuals. Using a stringent transgene detection assay, we demonstrate the potential of mass production of transgene free males.
通过对雄性进行性别分选并仅释放不育雄性，可以提高无菌昆虫技术（SIT）的现场性能。这可以通过通过形态学将雄性与雌性进行资源密集型分离来实现。或者，可以使用性别比例偏置遗传构建体有选择地去除一种性别，而无需手动或自动分选，但由此产生的基因工程（GE）控制剂将需要额外的政府监管。在这里，我们描述并证明了一种批量生产非 GE 雄性的遗传方法。该方法可用于在具有染色体性别决定的任何生物体中产生异配子性别（XY 或 WZ）。我们在超过 103 个人的批量培养物中观察到高达 100％的性别选择。使用严格的转基因检测测定法，我们证明了大量生产无转基因雄性的潜力。
