Sumimoto K, Inagaki K, Ito H, Marubayashi S, Yamada K, Kawasaki T, Dohi K
Department of Surgery, Hiroshima University School of Medicine, Japan.
Surgery. 1987 Nov;102(5):821-7.
This study was undertaken to determine whether pretreatment of the donor rat with coenzyme Q10 (CoQ10) would protect against hepatic ischemia induced for 30 minutes at normothermic body temperature. Fresh liver transplants were used as controls (minus warm ischemia of 30 minutes) and gave a 1-week survival rate of 84.6%. CoQ10 was administered intravenously (10 mg/kg body weight) to the donor rat 1 hour before induction of warm ischemia (group A). In another group (B), the same dose was given intravenously not only to the donor rat but also to the recipient rat 1 hour before grafting. None of the placebo group survived more than 2 days. The 1-week survival rates of the groups pretreated with CoQ10 were 45.5% for group A and 50% for group B. There was no significant difference between groups A and B. A statistically significant difference was demonstrated between the placebo group and both CoQ10-treated groups (p less than 0.05). It was therefore assumed that CoQ10, accumulated in the donor liver, was a primary factor in improving survival. Serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), serum alkaline phosphatase (SALP), total bilirubin, and total protein were measured by means of light and electron microscopic examination of the liver 6 months after transplantation. Long-term-surviving rats with transplanted, ischemically damaged liver that was pretreated with CoQ10 showed a decrease in the activity of SGOT and SGPT and an increase in levels of total protein to the normal range (as well as to those levels exhibited by fresh-liver-transplanted rats) with practically no change in levels of SALP, total bilirubin, or in histologic findings. These results indicate that donor pretreatment with CoQ10 is useful for increasing survival after warm ischemic damage of rat liver grafts.
本研究旨在确定用辅酶Q10（CoQ10）对供体大鼠进行预处理是否能预防在正常体温下诱导的30分钟肝脏缺血。新鲜肝脏移植用作对照（无30分钟的热缺血），1周存活率为84.6%。在诱导热缺血前1小时，给供体大鼠静脉注射CoQ10（10mg/kg体重）（A组）。在另一组（B组）中，在移植前1小时，不仅给供体大鼠而且给受体大鼠静脉注射相同剂量。安慰剂组无一存活超过2天。CoQ10预处理组的1周存活率，A组为45.5%，B组为50%。A组和B组之间无显著差异。安慰剂组与两个CoQ10治疗组之间存在统计学显著差异（p小于0.05）。因此可以认为，积聚在供体肝脏中的CoQ10是提高存活率的主要因素。在移植后6个月，通过肝脏的光镜和电镜检查，测量血清谷草转氨酶（SGOT）、血清谷丙转氨酶（SGPT）、血清碱性磷酸酶（SALP）、总胆红素和总蛋白。长期存活的大鼠，其移植的、经CoQ10预处理的缺血损伤肝脏，SGOT和SGPT活性降低，总蛋白水平升高至正常范围（以及新鲜肝脏移植大鼠所表现的水平），而SALP、总胆红素水平或组织学结果几乎没有变化。这些结果表明，用CoQ10对供体进行预处理有助于提高大鼠肝脏移植热缺血损伤后的存活率。
