Méndez Valentina, Valenzuela Miryam, Salvà-Serra Francisco, Jaén-Luchoro Daniel, Besoain Ximena, Moore Edward R B, Seeger Michael
Molecular Microbiology and Environmental Biotechnology Laboratory, Department of Chemistry and Centro de Biotecnología Daniel Alkalay Lowitt, Universidad Técnica Federico Santa María, Valparaíso 2390123, Chile.
Department of Infectious Diseases, Institute for Biomedicine, Sahlgrenska Academy, University of Gothenburg, 413 46 Gothenburg, Sweden.
Microorganisms. 2020 Oct 29;8(11):1679. doi: 10.3390/microorganisms8111679.
The genus has been associated largely with plant diseases. The aims of this study were to characterize the genomes and the virulence factors of Chilean subsp. strains VL527, MSF322 and OP3, and to define their phylogenomic positions within the species, . VL527 and MSF322 genomes possess 3,396,632 and 3,399,199 bp, respectively, with a pCM2-like plasmid in strain VL527, with pCM1- and pCM2-like plasmids in strain MSF322. OP3 genome is composed of a chromosome and three plasmids (including pCM1- and pCM2-like plasmids) of 3,466,104 bp. Genomic analyses confirmed the phylogenetic relationships of the Chilean strains among subsp. and showed their low genomic diversity. Different virulence levels in tomato plants were observable. Phylogenetic analyses of the virulence factors revealed that the gene ( region)-that grouped Chilean strains in three distinct clusters-and proteases and hydrolases encoding genes, exclusive for each of the Chilean strains, may be involved in these observed virulence levels. Based on genomic similarity (ANIm) analyses, a proposal to combine and reclassify subsp. and subsp. at the species level, as sp. nov., as well as to reclassify subsp. as the species . sp. nov. may be justified.
该属主要与植物病害相关。本研究的目的是对智利亚种菌株VL527、MSF322和OP3的基因组及毒力因子进行表征，并确定它们在该物种内的系统发育位置。VL527和MSF322基因组分别拥有3,396,632 bp和3,399,199 bp，VL527菌株含有一个类似pCM2的质粒，MSF322菌株含有类似pCM1和pCM2的质粒。OP3基因组由一条染色体和三个质粒（包括类似pCM1和pCM2的质粒）组成，大小为3,466,104 bp。基因组分析证实了智利菌株在亚种内的系统发育关系，并显示出它们较低的基因组多样性。在番茄植株中可观察到不同的毒力水平。对毒力因子的系统发育分析表明，将智利菌株分为三个不同簇的基因（区域）以及每个智利菌株特有的蛋白酶和水解酶编码基因可能与观察到的这些毒力水平有关。基于基因组相似性（ANIm）分析，有提议在物种水平上将亚种和亚种合并并重新分类为新物种，以及将亚种重新分类为物种。新物种可能是合理的。
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