不同剂量射干水提取物对大鼠慢性支气管炎发病相关因素的影响。
The Effect of Different Water Extracts from on Selected Factors Associated with Pathogenesis of Chronic Bronchitis in Rats.
机构信息
Department of Botany, Breeding and Agricultural Technology of Medicinal Plants, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska, Poland.
Department of Pharmacology, Poznań University of Medical Sciences, Rokietnicka 5a, 60-806 Poznań, Poland.
出版信息
Molecules. 2020 Oct 29;25(21):5020. doi: 10.3390/molecules25215020.
The aim of this study was to assess the activity of extracts from A. DC () in a model of chronic bronchitis in rats. The research was carried out on three water extracts: E1 - from roots of field cultivated ; E2 - from biotransformed roots of ; E3 - from callus of The extracts differed in saponins and inulin levels-the highest was measured in E3 and the lowest in E1. Identification of secondary metabolites was performed using two complementary LC-MS systems. Chronic bronchitis was induced by sodium metabisulfite (a source of SO). Animals were treated with extracts for three weeks (100 mg/kg, intragastrically) and endothelial growth factor (VEGF), transforming growth factors (TGF-β1, -β2, -β3), and mucin 5AC (MUC5AC) levels were determined in bronchoalveolar lavage fluid, whereas C reactive protein (CRP) level was measured in serum. Moreover, mRNA expression were assessed in bronchi and lungs. In SO-exposed rats, an elevation of the CRP, TGF-β1, TGF-β2, VEGF, and mucin was found, but the extracts' administration mostly reversed this phenomenon, leading to control values. The results showed a strong anti-inflammatory effect of the extracts from .
本研究旨在评估 A. DC()提取物在大鼠慢性支气管炎模型中的活性。研究采用三种水提取物进行：E1-来自田间种植的根；E2-来自生物转化的根；E3-来自愈伤组织。提取物在皂苷和菊粉水平上存在差异-E3 中的含量最高，E1 中的含量最低。使用两种互补的 LC-MS 系统进行了次生代谢产物的鉴定。慢性支气管炎由偏亚硫酸钠（SO 的来源）诱导。动物用提取物治疗 3 周（100mg/kg，灌胃），测定支气管肺泡灌洗液中内皮生长因子（VEGF）、转化生长因子（TGF-β1、-β2、-β3）和粘蛋白 5AC（MUC5AC）水平，血清中 C 反应蛋白（CRP）水平。此外，还评估了支气管和肺部的 mRNA 表达。在 SO 暴露的大鼠中，发现 CRP、TGF-β1、TGF-β2、VEGF 和粘蛋白升高，但提取物的给药大多逆转了这种现象，使这些指标恢复到正常水平。研究结果表明，A. DC 的提取物具有很强的抗炎作用。
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