Kim Ji Young, Hwang Yong Pil, Kim Dong Hee, Han Eun Hee, Chung Young Chul, Roh Seong Hwan, Jeong Hye Gwang
Department of Pharmacy, College of Pharmacy, Research Center for Proteineous Materials, Chosun University, Kwangju, South Korea.
Biosci Biotechnol Biochem. 2006 Apr;70(4):858-64. doi: 10.1271/bbb.70.858.
Previous studies have reported that the saponins isolated from the roots of Platycodon grandiflorum A. DC (Campanulaceae), Changkil saponins (CKS), inhibited cyclooxygenase-2 (COX-2) expression in cultured lipopolysaccharide-activated macrophages. The aim of this presented study was to confirm the anti-inflammatory effects of CKS by examining their effect on the inflammatory response induced by carrageenan in a rat by using an acute air pouch inflammation model. CKS significantly reduced the levels of the inflammatory process markers in the air pouch, such as the volume of exudates, the amount of protein and the number of leukocytes and neutrophils. The levels of TNF-alpha and prostaglandin E2 (PGE2) were also markedly lower in the air pouch of the CKS-treated animals than in the controls. An immunoblot analysis showed that CKS reduced the COX-2 expression level in the exudate cells. In addition, CKS significantly reduced the paw edema induced by carrageenan and also markedly reduced the level of PGE2 production in the inflamed paw. These results suggest that CKS had significant anti-inflammatory effects in vivo.
先前的研究报道，从桔梗（桔梗科）根部分离出的皂苷，长基尔皂苷（CKS），可抑制培养的脂多糖激活巨噬细胞中环氧合酶-2（COX-2）的表达。本研究的目的是通过使用急性气袋炎症模型，检测CKS对角叉菜胶诱导的大鼠炎症反应的影响，以证实其抗炎作用。CKS显著降低了气袋中炎症过程标志物的水平，如渗出液体积、蛋白量以及白细胞和中性粒细胞数量。与对照组相比，CKS处理动物气袋中肿瘤坏死因子-α（TNF-α）和前列腺素E2（PGE2）的水平也显著降低。免疫印迹分析表明，CKS降低了渗出细胞中COX-2的表达水平。此外，CKS显著减轻了角叉菜胶诱导的爪部水肿，并且也显著降低了炎症爪部中PGE2的产生水平。这些结果表明，CKS在体内具有显著的抗炎作用。
