Department of Anesthesia, Shanghai East Hospital, Tongji University School of Medicine , Shanghai, China.
Bioengineered. 2020 Dec;11(1):1269-1279. doi: 10.1080/21655979.2020.1832343.
Emerging evidence suggested that circular RNAs (circRNAs) play critical roles in cervical cancer (CC) progression. However, the roles and molecular mechanisms of hsa_circ_0007364 in the tumorigenesis of CC remain unclear. In the present study, we used bioinformatics analysis and a series of experimental analysis to characterize a novel circRNA, hsa_circ_0007364 was up-regulated and associated with advanced clinical features in CC patients. Hsa_circ_0007364 inhibition notably suppressed the proliferation and invasion abilities of CC cells in vitro and reduced tumor growth in vivo. Moreover, hsa_circ_0007364 was uncovered to sponge miR-101-5p. Additionally, methionine adenosyltransferase II alpha (MAT2A) was verified as a target gene of miR-101-5p, and its downregulation reversed the inhibitory effects of hsa_circ_0007364 knockdown on CC progression. Therefore, we suggested that hsa_circ_0007364 might serve as an oncogenic circRNA in CC progression by regulating the miR-101-5p/MAT2A axis, which provides a potential therapeutic target to the treatment. hsa_circ_0007364 was upregulated in CC hsa_circ_0007364 promoted CC cell progression hsa_circ_0007364/miR-101-5p/MAT2A axis in CC.
越来越多的证据表明，环状 RNA（circRNA）在宫颈癌（CC）进展中发挥着关键作用。然而，hsa_circ_0007364 在 CC 肿瘤发生中的作用和分子机制仍不清楚。在本研究中，我们使用生物信息学分析和一系列实验分析来描述一种新型的环状 RNA，hsa_circ_0007364 在 CC 患者中上调，并与晚期临床特征相关。hsa_circ_0007364 抑制显著抑制了 CC 细胞在体外的增殖和侵袭能力，并减少了体内肿瘤的生长。此外，hsa_circ_0007364 被揭示能海绵吸附 miR-101-5p。此外，甲硫氨酸腺苷转移酶 IIα（MAT2A）被验证为 miR-101-5p 的靶基因，其下调逆转了 hsa_circ_0007364 敲低对 CC 进展的抑制作用。因此，我们认为 hsa_circ_0007364 可能通过调节 miR-101-5p/MAT2A 轴在 CC 进展中发挥致癌环状 RNA 的作用，为治疗提供了一个潜在的治疗靶点。
