Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712.
Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712.
Proc Natl Acad Sci U S A. 2020 Nov 17;117(46):29178-29189. doi: 10.1073/pnas.2018735117. Epub 2020 Nov 2.
Ethylene is an important plant hormone that regulates plant growth, in which the master transcriptionactivator EIN3 (Ethylene Insensitive 3)-mediated transcriptional activation plays vital roles. However, the EIN3-mediated transcriptional repression in ethylene response is unknown. We report here that a Transcriptional Repressor of EIN3-dependent Ethylene-response 1 (TREE1) interacts with EIN3 to regulate transcriptional repression that leads to an inhibition of shoot growth in response to ethylene. Tissue-specific transcriptome analysis showed that most of the genes are down-regulated by ethylene in shoots, and a DNA binding motif was identified that is important for this transcriptional repression. TREE1 binds to the DNA motif to repress gene expression in an EIN3-dependent manner. Genetic validation demonstrated that repression of TREE1-targeted genes leads to an inhibition of shoot growth. Overall, this work establishes a mechanism by which transcriptional repressor TREE1 interacts with EIN3 to inhibit shoot growth via transcriptional repression in response to ethylene.
乙烯是一种重要的植物激素，调节植物生长，其中主转录激活因子 EIN3（乙烯不敏感 3）介导的转录激活起着至关重要的作用。然而，乙烯反应中 EIN3 介导的转录抑制作用尚不清楚。我们在这里报告，转录抑制因子 EIN3 依赖的乙烯反应 1（TREE1）与 EIN3 相互作用，调节转录抑制，导致对乙烯的芽生长抑制。组织特异性转录组分析表明，大多数基因在芽中被乙烯下调，并且鉴定出一个对这种转录抑制很重要的 DNA 结合基序。TREE1 结合到 DNA 基序上，以 EIN3 依赖的方式抑制基因表达。遗传验证表明，抑制 TREE1 靶向基因会导致芽生长抑制。总的来说，这项工作建立了一个机制，即转录抑制因子 TREE1 与 EIN3 相互作用，通过转录抑制抑制芽生长，以响应乙烯。
