Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712, USA.
Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA.
Sci Adv. 2024 Jul 26;10(30):eado2825. doi: 10.1126/sciadv.ado2825.
Ethylene plays its essential roles in plant development, growth, and defense responses by controlling the transcriptional reprograming, in which EIN2-C-directed regulation of histone acetylation is the first key step for chromatin to perceive ethylene signaling. But how the nuclear acetyl coenzyme A (acetyl CoA) is produced to ensure the ethylene-mediated histone acetylation is unknown. Here we report that ethylene triggers the accumulation of the pyruvate dehydrogenase complex (PDC) in the nucleus to synthesize nuclear acetyl CoA to regulate ethylene response. PDC is identified as an EIN2-C nuclear partner, and ethylene triggers its nuclear accumulation. Mutations in PDC lead to an ethylene hyposensitivity that results from the reduction of histone acetylation and transcription activation. Enzymatically active nuclear PDC synthesizes nuclear acetyl CoA for EIN2-C-directed histone acetylation and transcription regulation. These findings uncover a mechanism by which PDC-EIN2 converges the mitochondrial enzyme-mediated nuclear acetyl CoA synthesis with epigenetic and transcriptional regulation for plant hormone response.
乙烯通过控制转录重编程在植物发育、生长和防御反应中发挥重要作用，其中 EIN2-C 指导的组蛋白乙酰化调控是染色质感知乙烯信号的第一步关键步骤。但是，核乙酰辅酶 A（acetyl CoA）是如何产生的，以确保乙烯介导的组蛋白乙酰化尚不清楚。在这里，我们报告乙烯触发丙酮酸脱氢酶复合物（PDC）在核内积累以合成核乙酰辅酶 A 来调节乙烯反应。PDC 被鉴定为 EIN2-C 的核伴侣，乙烯触发其核积累。PDC 的突变导致乙烯反应迟钝，这是由于组蛋白乙酰化和转录激活减少所致。具有酶活性的核 PDC 合成核乙酰辅酶 A，用于 EIN2-C 指导的组蛋白乙酰化和转录调控。这些发现揭示了一种机制，即 PDC-EIN2 将线粒体酶介导的核乙酰辅酶 A 合成与植物激素反应的表观遗传和转录调控相融合。
