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基于 DNA 的细胞器电压表。

A DNA-based voltmeter for organelles.

机构信息

Department of Chemistry, The University of Chicago, Chicago, IL, USA.

Grossman Institute of Neuroscience, Quantitative Biology and Human Behavior, The University of Chicago, Chicago, IL, USA.

出版信息

Nat Nanotechnol. 2021 Jan;16(1):96-103. doi: 10.1038/s41565-020-00784-1. Epub 2020 Nov 2.

DOI:10.1038/s41565-020-00784-1
PMID:33139937
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8513801/
Abstract

The role of membrane potential in most intracellular organelles remains unexplored because of the lack of suitable tools. Here, we describe Voltair, a fluorescent DNA nanodevice that reports the absolute membrane potential and can be targeted to organelles in live cells. Voltair consists of a voltage-sensitive fluorophore and a reference fluorophore for ratiometry, and acts as an endocytic tracer. Using Voltair, we could measure the membrane potential of different organelles in situ in live cells. Voltair can potentially guide the rational design of biocompatible electronics and enhance our understanding of how membrane potential regulates organelle biology.

摘要

由于缺乏合适的工具，大多数细胞内细胞器的膜电位作用仍未得到探索。在这里，我们描述了Voltair，一种荧光 DNA 纳米器件，可报告绝对膜电位，并可靶向活细胞中的细胞器。Voltair 由一个电压敏感荧光团和一个用于比率测量的参比荧光团组成，并且可以作为内吞追踪剂。使用 Voltair，我们可以在活细胞中实时测量不同细胞器的膜电位。Voltair 有可能指导生物相容性电子设备的合理设计，并增强我们对膜电位如何调节细胞器生物学的理解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b2/8513801/03e2d582f7cb/nihms-1631136-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b2/8513801/f08b0a8a71ed/nihms-1631136-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b2/8513801/3f46d9305182/nihms-1631136-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b2/8513801/4d099ff6e540/nihms-1631136-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b2/8513801/1175b1b6f3d5/nihms-1631136-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b2/8513801/03e2d582f7cb/nihms-1631136-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b2/8513801/f08b0a8a71ed/nihms-1631136-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b2/8513801/3f46d9305182/nihms-1631136-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b2/8513801/4d099ff6e540/nihms-1631136-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b2/8513801/1175b1b6f3d5/nihms-1631136-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b2/8513801/03e2d582f7cb/nihms-1631136-f0004.jpg

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