细胞器膜电位成像的荧光指示剂。
Fluorescent indicators for imaging membrane potential of organelles.
机构信息
Department of Chemistry, University of California, Berkeley, CA, 94720, USA.
Department of Chemistry, University of California, Berkeley, CA, 94720, USA; Department of Molecular & Cell Biology, University of California, Berkeley, CA, 94720, USA; Helen Wills Neuroscience Institute, University of California, Berkeley, CA, 94720, USA.
出版信息
Curr Opin Chem Biol. 2022 Dec;71:102203. doi: 10.1016/j.cbpa.2022.102203. Epub 2022 Sep 6.
Plasma membrane potential is a key driver of the physiology of excitable cells like neurons and cardiomyocytes. Voltage-sensitive fluorescent indicators offer a powerful complement to traditional electrode-based approaches to measuring and monitoring membrane potential. Intracellular organelles can also generate membrane potential, yet the electrode- and fluorescent indicator-based approaches used for plasma membrane potential imaging are difficult to implement on intact organelles in their native environment. Here, we survey recent advances in developing and deploying voltage-sensitive fluorescent indicators to interrogate organelle membrane potential in intact cells.
质膜电位是神经元和心肌细胞等可兴奋细胞生理学的关键驱动因素。电压敏感荧光指示剂为测量和监测膜电位的传统基于电极的方法提供了有力的补充。细胞内细胞器也可以产生膜电位，但是用于质膜电位成像的基于电极和荧光指示剂的方法很难在其天然环境中对完整细胞器进行实施。在这里，我们调查了开发和部署电压敏感荧光指示剂以在完整细胞中检测细胞器膜电位的最新进展。
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